WANG Jing; LV XiaoWen; SHI JiePing; HU XiaoSong; DU YuGuo

doi:10.3967/0895-3988.2011.04.010

Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(4): 391-399

WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010

Citation:

WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010

doi: 10.3967/0895-3988.2011.04.010

基金项目: the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)

Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Funds: the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)

摘要: Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARY and POX-induced ROS were explored.Methods [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR & RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARY pathway and POX-induced ROS formation in HT29 cells.Results Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis.During this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARY.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARY-dependent pattern.Furthermore,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARy-dependent apoptosis induced by troglitazone.Conclusion The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARY activation.
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Abstract: Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARY and POX-induced ROS were explored.Methods [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR & RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARY pathway and POX-induced ROS formation in HT29 cells.Results Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis.During this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARY.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARY-dependent pattern.Furthermore,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARy-dependent apoptosis induced by troglitazone.Conclusion The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARY activation.
- Troglitazone /
- Apoptosis /
- HT29 /
- POX /
- ROS formation /
- PPARY /
- Cytochrome c release

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Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

doi: 10.3967/0895-3988.2011.04.010

基金项目: the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)

刊出日期: 2011-08-20

关键词:

Troglitazone /

Apoptosis /

HT29 /

POX /

ROS formation /

PPARY /

Cytochrome c release

摘要: Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARY and POX-induced ROS were explored.Methods [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR & RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARY pathway and POX-induced ROS formation in HT29 cells.Results Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis.During this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARY.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARY-dependent pattern.Furthermore,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARy-dependent apoptosis induced by troglitazone.Conclusion The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARY activation.

English Abstract

Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China

Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Funds: the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)

Publish Date: 2011-08-20

Keywords:

Abstract: Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARY and POX-induced ROS were explored.Methods [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR & RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARY pathway and POX-induced ROS formation in HT29 cells.Results Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis.During this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARY.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARY-dependent pattern.Furthermore,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARy-dependent apoptosis induced by troglitazone.Conclusion The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARY activation.

Citation: