Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

WANG Jing LV XiaoWen SHI JiePing HU XiaoSong DU YuGuo

WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010
Citation: WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010

doi: 10.3967/0895-3988.2011.04.010
基金项目: the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)

Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

Funds: the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)
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  • 刊出日期:  2011-08-20

Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

doi: 10.3967/0895-3988.2011.04.010
    基金项目:  the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)

摘要: Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARY and POX-induced ROS were explored.Methods [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR & RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARY pathway and POX-induced ROS formation in HT29 cells.Results Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis.During this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARY.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARY-dependent pattern.Furthermore,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARy-dependent apoptosis induced by troglitazone.Conclusion The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARY activation.

English Abstract

WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010
Citation: WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010

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