Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the Expression of SET-Associated Proteins in Human Hepatic L-02 Cells

HONG Wen Xu YE Jin Bo CHEN Mou Tong YAN Yan ZHOU Gui Feng YANG Xi Fei YANG Liang

HONG Wen Xu, YE Jin Bo, CHEN Mou Tong, YAN Yan, ZHOU Gui Feng, YANG Xi Fei, YANG Liang. Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the Expression of SET-Associated Proteins in Human Hepatic L-02 Cells[J]. Biomedical and Environmental Sciences, 2013, 26(7): 618-621. doi: 10.3967/0895-3988.2013.07.016
Citation: HONG Wen Xu, YE Jin Bo, CHEN Mou Tong, YAN Yan, ZHOU Gui Feng, YANG Xi Fei, YANG Liang. Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the Expression of SET-Associated Proteins in Human Hepatic L-02 Cells[J]. Biomedical and Environmental Sciences, 2013, 26(7): 618-621. doi: 10.3967/0895-3988.2013.07.016

doi: 10.3967/0895-3988.2013.07.016

Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the Expression of SET-Associated Proteins in Human Hepatic L-02 Cells

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  • 刊出日期:  2013-07-20

Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the Expression of SET-Associated Proteins in Human Hepatic L-02 Cells

doi: 10.3967/0895-3988.2013.07.016

摘要: Trichloroethylene (TCE) is a major pollutant that affects both occupational and general environments[1].The liver is an important target organ of TCE[1].Substantial efforts and remarkable progress into understanding TCE cytotoxicity have been made in cultured liver cells.However,the molecular mechanisms by which TCE induces hepatotoxicity are not well understood.SET (also known as protein phosphatase 2A inhibitor,I2PP2A,or template-activating factor-Ⅰ,TAF-Ⅰ) is a nuclear protein that regulates histone modification,gene transcription,DNA replication,nucleosome assembly,phosphatase activity and kinase activity[2].Previously,we observed a significant TCE-induced up-regulation of SET in human hepatic L-02 cells[3].We further reported that the knockdown of SET significantly attenuated TCE-induced cytotoxicity,rescued the inhibition of PP2A activity,and suppressed the activation of caspase-3 mediated by TCE treatment in L-02 cells.Recently,we identified 42 novel Gene Ontology (GO) co-annotated SET-binding proteins and revealed the effects of TCE on the distribution and interactions of SET and its binding partners,eEF1A1 and eEF1A2,in L-02 cells[4].Herein,we describe a biphasic concentration-response in both cell proliferation and the expression of SET-associated proteins to TCE treatment of L-02 cells.Our results suggest that the TCE-induced alterations in the expression of SET and its binding partners may be involved in the biphasic effect induced by TCE and partially constitute the mechanisms leading to TCE hepatotoxicity.

English Abstract

HONG Wen Xu, YE Jin Bo, CHEN Mou Tong, YAN Yan, ZHOU Gui Feng, YANG Xi Fei, YANG Liang. Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the Expression of SET-Associated Proteins in Human Hepatic L-02 Cells[J]. Biomedical and Environmental Sciences, 2013, 26(7): 618-621. doi: 10.3967/0895-3988.2013.07.016
Citation: HONG Wen Xu, YE Jin Bo, CHEN Mou Tong, YAN Yan, ZHOU Gui Feng, YANG Xi Fei, YANG Liang. Trichloroethylene Induces Biphasic Concentration-dependent Changes in Cell Proliferation and the Expression of SET-Associated Proteins in Human Hepatic L-02 Cells[J]. Biomedical and Environmental Sciences, 2013, 26(7): 618-621. doi: 10.3967/0895-3988.2013.07.016

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