WANG An Na; WANG Quan Kai; YANG Min; HU Jie; DONG Lin; andXU Jian Ning

doi:10.3967/bes2014.002

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

WANG An Na, WANG Quan Kai, YANG Min, HU Jie, DONG Lin, andXU Jian Ning

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(7): 523-530

WANG An Na, WANG Quan Kai, YANG Min, HU Jie, DONG Lin, andXU Jian Ning. Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation[J]. Biomedical and Environmental Sciences, 2014, 27(7): 523-530. doi: 10.3967/bes2014.002

Citation:

WANG An Na, WANG Quan Kai, YANG Min, HU Jie, DONG Lin, andXU Jian Ning. Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation[J]. Biomedical and Environmental Sciences, 2014, 27(7): 523-530. doi: 10.3967/bes2014.002

doi: 10.3967/bes2014.002

基金项目: National Natural Science Foundation of China(81072318)

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

National Institute of 0ccupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Funds: National Natural Science Foundation of China(81072318)

摘要: ObjectiveTo establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA)and define the different methylation genes at different stages. MethodsDNA was extracted at different 16HBE malignant phasesandchanges of genes DNA methylation atdifferent stages weredetectedusing Methylation chip of‘NimbleGen HG18 CpG Promoter Microarray Methylation’. Methylation-specific PCR (MSP) was usedto observe the methylation status ofsome genes, and then compared with the control groups. ResultsThe resultshowed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes inmetaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not inmetaphase and anaphase; 272 genes in metaphase but not inprotophase and anaphase; 683 genes in anaphase but not inmetaphase and protophase; 73 genes inprotophase andmetaphase but not in anaphase; 67 genes in protophase and anaphase but not inmetaphase; 59 genes inmetaphase and anaphase but not in protophase. ConclusionThe pattern of DNA methylation could change in the process of 16HBEinduced by GMA.
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Abstract: ObjectiveTo establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA)and define the different methylation genes at different stages. MethodsDNA was extracted at different 16HBE malignant phasesandchanges of genes DNA methylation atdifferent stages weredetectedusing Methylation chip of‘NimbleGen HG18 CpG Promoter Microarray Methylation’. Methylation-specific PCR (MSP) was usedto observe the methylation status ofsome genes, and then compared with the control groups. ResultsThe resultshowed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes inmetaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not inmetaphase and anaphase; 272 genes in metaphase but not inprotophase and anaphase; 683 genes in anaphase but not inmetaphase and protophase; 73 genes inprotophase andmetaphase but not in anaphase; 67 genes in protophase and anaphase but not inmetaphase; 59 genes inmetaphase and anaphase but not in protophase. ConclusionThe pattern of DNA methylation could change in the process of 16HBEinduced by GMA.
- DNA methylation /
- Chip /
- Glycidyl methacrylate /
- 16HBE

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Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

doi: 10.3967/bes2014.002

基金项目: National Natural Science Foundation of China(81072318)

刊出日期: 2014-07-20

关键词:

DNA methylation /

Chip /

Glycidyl methacrylate /

16HBE

摘要: ObjectiveTo establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA)and define the different methylation genes at different stages. MethodsDNA was extracted at different 16HBE malignant phasesandchanges of genes DNA methylation atdifferent stages weredetectedusing Methylation chip of‘NimbleGen HG18 CpG Promoter Microarray Methylation’. Methylation-specific PCR (MSP) was usedto observe the methylation status ofsome genes, and then compared with the control groups. ResultsThe resultshowed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes inmetaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not inmetaphase and anaphase; 272 genes in metaphase but not inprotophase and anaphase; 683 genes in anaphase but not inmetaphase and protophase; 73 genes inprotophase andmetaphase but not in anaphase; 67 genes in protophase and anaphase but not inmetaphase; 59 genes inmetaphase and anaphase but not in protophase. ConclusionThe pattern of DNA methylation could change in the process of 16HBEinduced by GMA.

English Abstract

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

National Institute of 0ccupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Funds: National Natural Science Foundation of China(81072318)

Publish Date: 2014-07-20

Keywords:

Abstract: ObjectiveTo establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA)and define the different methylation genes at different stages. MethodsDNA was extracted at different 16HBE malignant phasesandchanges of genes DNA methylation atdifferent stages weredetectedusing Methylation chip of‘NimbleGen HG18 CpG Promoter Microarray Methylation’. Methylation-specific PCR (MSP) was usedto observe the methylation status ofsome genes, and then compared with the control groups. ResultsThe resultshowed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes inmetaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not inmetaphase and anaphase; 272 genes in metaphase but not inprotophase and anaphase; 683 genes in anaphase but not inmetaphase and protophase; 73 genes inprotophase andmetaphase but not in anaphase; 67 genes in protophase and anaphase but not inmetaphase; 59 genes inmetaphase and anaphase but not in protophase. ConclusionThe pattern of DNA methylation could change in the process of 16HBEinduced by GMA.

Citation:

WANG An Na, WANG Quan Kai, YANG Min, HU Jie, DONG Lin, andXU Jian Ning. Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation[J]. Biomedical and Environmental Sciences, 2014, 27(7): 523-530. doi: 10.3967/bes2014.002

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doi: 10.3967/bes2014.002

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

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Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

doi: 10.3967/bes2014.002

English Abstract

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

National Institute of 0ccupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China

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