ZHANG Jun; ZHANG Jing; LI MengJie; Wu YouJun; FAN YingYing; ZHOU Yang; TAN LiLi; SHAO ZhiHua; SHI HongJun

doi:10.3967/0895-3988.2011.02.011

Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

ZHANG Jun, ZHANG Jing, LI MengJie, Wu YouJun, FAN YingYing, ZHOU Yang, TAN LiLi, SHAO ZhiHua, SHI HongJun

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(2): 163-171

ZHANG Jun, ZHANG Jing, LI MengJie, Wu YouJun, FAN YingYing, ZHOU Yang, TAN LiLi, SHAO ZhiHua, SHI HongJun. Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats[J]. Biomedical and Environmental Sciences, 2011, 24(2): 163-171. doi: 10.3967/0895-3988.2011.02.011

Citation:

ZHANG Jun, ZHANG Jing, LI MengJie, Wu YouJun, FAN YingYing, ZHOU Yang, TAN LiLi, SHAO ZhiHua, SHI HongJun. Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats[J]. Biomedical and Environmental Sciences, 2011, 24(2): 163-171. doi: 10.3967/0895-3988.2011.02.011

doi: 10.3967/0895-3988.2011.02.011

基金项目: the Natural Science Foundation of China(30570690%81041069)%the Natural Science Foundation of Shanghai(08ZR1420700)

Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China
School of Life Science and Technology, Tongji University, Shanghai 200092, China

Funds: the Natural Science Foundation of China(30570690%81041069)%the Natural Science Foundation of Shanghai(08ZR1420700)

摘要: Objective To investigate the expression variation of RAR-β2, RASSF1A, and CDKN2A gene in the process of nickel-induced carcinogenesis. Methods Nickel subsulfide (Ni<,3>S<,2>) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real-time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real-time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR-β2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure.
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Abstract: Objective To investigate the expression variation of RAR-β2, RASSF1A, and CDKN2A gene in the process of nickel-induced carcinogenesis. Methods Nickel subsulfide (Ni<,3>S<,2>) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real-time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real-time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR-β2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure.
- Nickel /
- DNA methylation /
- RAR-β2 /
- RASSF1A /
- CDKN2A /
- Quantitative Real-time Methylation Specific PCR

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Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

doi: 10.3967/0895-3988.2011.02.011

基金项目: the Natural Science Foundation of China(30570690%81041069)%the Natural Science Foundation of Shanghai(08ZR1420700)

刊出日期: 2011-04-20

关键词:

Nickel /

DNA methylation /

RAR-β2 /

RASSF1A /

CDKN2A /

Quantitative Real-time Methylation Specific PCR

摘要: Objective To investigate the expression variation of RAR-β2, RASSF1A, and CDKN2A gene in the process of nickel-induced carcinogenesis. Methods Nickel subsulfide (Ni<,3>S<,2>) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real-time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real-time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR-β2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure.

English Abstract

Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China

School of Life Science and Technology, Tongji University, Shanghai 200092, China

Funds: the Natural Science Foundation of China(30570690%81041069)%the Natural Science Foundation of Shanghai(08ZR1420700)

Publish Date: 2011-04-20

Keywords:

Nickel /
DNA methylation /
RAR-β2 /
RASSF1A /
CDKN2A /
Quantitative Real-time Methylation Specific PCR

Abstract: Objective To investigate the expression variation of RAR-β2, RASSF1A, and CDKN2A gene in the process of nickel-induced carcinogenesis. Methods Nickel subsulfide (Ni<,3>S<,2>) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real-time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real-time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR-β2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure.

Citation:

ZHANG Jun, ZHANG Jing, LI MengJie, Wu YouJun, FAN YingYing, ZHOU Yang, TAN LiLi, SHAO ZhiHua, SHI HongJun. Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats[J]. Biomedical and Environmental Sciences, 2011, 24(2): 163-171. doi: 10.3967/0895-3988.2011.02.011

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doi: 10.3967/0895-3988.2011.02.011

Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

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出版历程

Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

doi: 10.3967/0895-3988.2011.02.011

English Abstract

Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China School of Life Science and Technology, Tongji University, Shanghai 200092, China

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Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China

School of Life Science and Technology, Tongji University, Shanghai 200092, China