TAN Hong Bo; WANG Fu You; DING Wei; ZHANG Ying; DING Jing; CAI Di Xin; YU Kai Fu; YANG Jun; YANG Liu; XU Yong Qing

doi:10.3967/bes2015.024

Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing*

TAN Hong Bo, WANG Fu You, DING Wei, ZHANG Ying, DING Jing, CAI Di Xin, YU Kai Fu, YANG Jun, YANG Liu, XU Yong Qing

文章导航 > Biomedical and Environmental Sciences > 2015 > 28(3): 178-189

TAN Hong Bo, WANG Fu You, DING Wei, ZHANG Ying, DING Jing, CAI Di Xin, YU Kai Fu, YANG Jun, YANG Liu, XU Yong Qing. Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing*[J]. Biomedical and Environmental Sciences, 2015, 28(3): 178-189. doi: 10.3967/bes2015.024

Citation:

TAN Hong Bo, WANG Fu You, DING Wei, ZHANG Ying, DING Jing, CAI Di Xin, YU Kai Fu, YANG Jun, YANG Liu, XU Yong Qing. Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing*[J]. Biomedical and Environmental Sciences, 2015, 28(3): 178-189. doi: 10.3967/bes2015.024

doi: 10.3967/bes2015.024

基金项目: Thisstudywas supported by thefinancial support ofthe National Science Foundation(Grant 81360274)%the Kunming General Hospital Project(2012YG12)

Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing*

Department of 0rthopedics, Kunming General Hospital ofChinesePeople’sLiberationArmy, DaguanRoad, Kunming 650032,Yunnan,China
Center for Joint Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China
Department of Clinical Laboratory, Kunming General Hospital,Chinese People’sLiberationArmy, DaguanRoad, Kunming 650032,Yunnan,China

Funds: Thisstudywas supported by thefinancial support ofthe National Science Foundation(Grant 81360274)%the Kunming General Hospital Project(2012YG12)

摘要: ObjectiveTo develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration. MethodsThe chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds. The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated. The antibacterial activity was tested by usingS. aureusandE. colisuspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds. ResultsThe adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan. KCS scaffolds could obviously decrease bacteria number.The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration. It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples. ConclusionThe porous KCS scaffold may be used as implanted scaffold materials forpromoting wound healing and skinregeneration.
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Abstract: ObjectiveTo develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration. MethodsThe chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds. The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated. The antibacterial activity was tested by usingS. aureusandE. colisuspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds. ResultsThe adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan. KCS scaffolds could obviously decrease bacteria number.The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration. It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples. ConclusionThe porous KCS scaffold may be used as implanted scaffold materials forpromoting wound healing and skinregeneration.
- Keratin /
- Chitosan /
- Porous scaffold /
- Wound healing /
- Skin regeneration

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Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing*

doi: 10.3967/bes2015.024

基金项目: Thisstudywas supported by thefinancial support ofthe National Science Foundation(Grant 81360274)%the Kunming General Hospital Project(2012YG12)

刊出日期: 2015-03-20

关键词:

Keratin /

Chitosan /

Porous scaffold /

Wound healing /

Skin regeneration

摘要: ObjectiveTo develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration. MethodsThe chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds. The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated. The antibacterial activity was tested by usingS. aureusandE. colisuspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds. ResultsThe adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan. KCS scaffolds could obviously decrease bacteria number.The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration. It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples. ConclusionThe porous KCS scaffold may be used as implanted scaffold materials forpromoting wound healing and skinregeneration.

English Abstract

Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing*

Department of 0rthopedics, Kunming General Hospital ofChinesePeople’sLiberationArmy, DaguanRoad, Kunming 650032,Yunnan,China

Center for Joint Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China

Department of Clinical Laboratory, Kunming General Hospital,Chinese People’sLiberationArmy, DaguanRoad, Kunming 650032,Yunnan,China

Funds: Thisstudywas supported by thefinancial support ofthe National Science Foundation(Grant 81360274)%the Kunming General Hospital Project(2012YG12)

Publish Date: 2015-03-20

Keywords:

Abstract: ObjectiveTo develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration. MethodsThe chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds. The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated. The antibacterial activity was tested by usingS. aureusandE. colisuspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds. ResultsThe adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan. KCS scaffolds could obviously decrease bacteria number.The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration. It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples. ConclusionThe porous KCS scaffold may be used as implanted scaffold materials forpromoting wound healing and skinregeneration.

Citation:

TAN Hong Bo, WANG Fu You, DING Wei, ZHANG Ying, DING Jing, CAI Di Xin, YU Kai Fu, YANG Jun, YANG Liu, XU Yong Qing. Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing*[J]. Biomedical and Environmental Sciences, 2015, 28(3): 178-189. doi: 10.3967/bes2015.024