Hanan Saleh; Amel M Soliman; Ayman S Mohamed; Mohamed-Assem S Marie

doi:10.3967/bes2015.082

Antioxidant Effect of Sepia Ink Extract on Extrahepatic Cholestasis Induced by Bile Duct Ligation in Rats

Hanan Saleh, Amel M Soliman, Ayman S Mohamed, Mohamed-Assem S Marie

文章导航 > Biomedical and Environmental Sciences > 2015 > 28(8): 582-594

Hanan Saleh, Amel M Soliman, Ayman S Mohamed, Mohamed-Assem S Marie. Antioxidant Effect of Sepia Ink Extract on Extrahepatic Cholestasis Induced by Bile Duct Ligation in Rats[J]. Biomedical and Environmental Sciences, 2015, 28(8): 582-594. doi: 10.3967/bes2015.082

Citation:

Hanan Saleh, Amel M Soliman, Ayman S Mohamed, Mohamed-Assem S Marie. Antioxidant Effect of Sepia Ink Extract on Extrahepatic Cholestasis Induced by Bile Duct Ligation in Rats[J]. Biomedical and Environmental Sciences, 2015, 28(8): 582-594. doi: 10.3967/bes2015.082

doi: 10.3967/bes2015.082

Antioxidant Effect of Sepia Ink Extract on Extrahepatic Cholestasis Induced by Bile Duct Ligation in Rats

Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt

摘要: Objective The aim of our study was to assess the complications of hepatic fibrosis associated with bile duct ligation and the potential curative role of sepia ink extract in hepatic damage induced by bile duct ligation.
Methods Rattus norvegicus rats were divided into 3 groups: Sham-operated group, model rats that underwent common bile duct ligation (BDL), and BDL rats treated orally with sepia ink extract (200 mg/kg body weight) for 7, 14, and 28 d after BDL.
Results There was a significant reduction in hepatic enzymes, ALP, GGT, bilirubin levels, and oxidative stress in the BDL group after treatment with sepia ink extract. Collagen deposition reduced after sepia ink extract treatment as compared to BDL groups, suggesting that the liver was repaired. Histopathological examination of liver treated with sepia ink extract showed moderate degeneration in the hepatic architecture and mild degeneration in hepatocytes as compared to BDL groups.
Conclusion Sepia ink extract provides a curative effect and an antioxidant capacity on BDL rats and could ameliorate the complications of liver cholestasis.
- /
- /
- /
- /
Abstract: Objective The aim of our study was to assess the complications of hepatic fibrosis associated with bile duct ligation and the potential curative role of sepia ink extract in hepatic damage induced by bile duct ligation.
Methods Rattus norvegicus rats were divided into 3 groups: Sham-operated group, model rats that underwent common bile duct ligation (BDL), and BDL rats treated orally with sepia ink extract (200 mg/kg body weight) for 7, 14, and 28 d after BDL.
Results There was a significant reduction in hepatic enzymes, ALP, GGT, bilirubin levels, and oxidative stress in the BDL group after treatment with sepia ink extract. Collagen deposition reduced after sepia ink extract treatment as compared to BDL groups, suggesting that the liver was repaired. Histopathological examination of liver treated with sepia ink extract showed moderate degeneration in the hepatic architecture and mild degeneration in hepatocytes as compared to BDL groups.
Conclusion Sepia ink extract provides a curative effect and an antioxidant capacity on BDL rats and could ameliorate the complications of liver cholestasis.
- Bile duct ligation /
- Hepatic fibrosis /
- Oxidative stress /
- Liver collagen percentage /
- Histopathological examination

[1]	Jinyu Fang, Huaicun Liu, Yanfei Zhang, Quancheng Cheng, Ziyuan Wang, Xuan Fang, Huiru Ding, Weiguang Zhang, Chunhua Chen. Nogo-A Protein Mediates Oxidative Stress and Synaptic Damage Induced by High-Altitude Hypoxia in the Rat Hippocampus . Biomedical and Environmental Sciences, 2025, 38(1): 79-93. doi: 10.3967/bes2024.112
[2]	GU Wen Fei, SHI Xia, MA Xin, YU Jun Lei, XU Jin Chuan, QIAN Cheng Cheng, HU Zhi Dong, ZHANG Hui. Effectiveness of Histopathological Examination of Ultrasound-guided Puncture Biopsy Samples for Diagnosis of Extrapulmonary Tuberculosis . Biomedical and Environmental Sciences, 2024, 37(2): 170-177. doi: 10.3967/bes2024.017
[3]	Miranda Laise Mara Oliveira, Agostini Lívia da Cunha, Lima Wanderson Geraldo de, Camini Fernanda Caetano, Costa Daniela Caldeira. Silymarin Attenuates Hepatic and Pancreatic Redox Imbalance Independent of Glycemic Regulation in the Alloxan-induced Diabetic Rat Model . Biomedical and Environmental Sciences, 2020, 33(9): 690-700. doi: 10.3967/bes2020.090
[4]	Hanen BOUAZIZ-KETATA, Ghada BEN SALAH, Hichem BEN SALAH, Rim MARREKCHI, Kamel JAMOUSSI, Tahia BOUDAWARA, Faiza FAKHFEKH, Najiba ZEGHAL. Nitrate-induced Biochemical and Histopathological Changes in the Liver of Rats:Ameliorative Effect of Hyparrhenia hirta . Biomedical and Environmental Sciences, 2014, 27(9): 695-706. doi: 10.3967/bes2014.105
[5]	Shikha Sharma, Ritu Gupta, Sonu Chand Thakur. Attenuation of Collagen Induced Arthritis by Centella asiatica Methanol Fraction via Modulation of Cytokines and Oxidative Stress . Biomedical and Environmental Sciences, 2014, 27(12): 926-938. doi: 10.3967/bes2014.133
[6]	YUAN Yan, BIAN Jian Chun, LIU Xue Zhong, ZHANG Ying, SUN Ya, LIU Zong Ping. Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro . Biomedical and Environmental Sciences, 2012, 25(2): 172-181. doi: 10.3967/0895-3988.2012.02.008
[7]	DONG CHANG, FAN WANG, YA-SHUANG ZHAO, HONG-ZHI PAN. Evaluation of Oxidative Stress in Colorectal Cancer Patients . Biomedical and Environmental Sciences, 2008, 21(4): 286-289.
[8]	RI-AN YU, TAO XIA, AI-GUO WANG, XUE-MIN CHEN. Effects of Selenium and Zinc on Renal Oxidative Stress and Apoptosis Induced by Fluoride in Rats . Biomedical and Environmental Sciences, 2006, 19(6): 439-444.
[9]	YOU-GEN ZHU, SHU-MEI ZHANG, JI-YUE WANG, WEI-QIANG XIAO, XIN-YU WANG, JUN-FU ZHOU. Overweight and Obesity-Induced Oxidative Stress in Children . Biomedical and Environmental Sciences, 2006, 19(5): 353-359.
[10]	JIAN-GUO LOU, JIE DONG, YI-CHUN ZHENG, SHU-MEI ZHANG, WEI-QIANG XIAO, JUN-FU ZHOU. Increased Oxidative Stress and Damage in Patients With Chronic Bacterial Prostatitis . Biomedical and Environmental Sciences, 2006, 19(6): 481-486.
[11]	JUN-FU ZHOU, XIN-YU WANG, XUE-JUN SHANGGUAN, ZU-MING GAO, SHU-MEI ZHANG, WEI-QIANG XIAO, CHANG-GUI CHEN. Increased Oxidative Stress in Women With Pregnancy-induced Hypertension . Biomedical and Environmental Sciences, 2005, 18(6): 419-426.
[12]	JUN-FU ZHOU, WEN ZHOU, SHU-MEI ZHANG, YAN-ER LUO, Huai-Hong Chen. Oxidative Stress and Free Radical Damage in Patients With Acute Dipterex Poisoning . Biomedical and Environmental Sciences, 2004, 17(2): 223-233.
[13]	YOU-GEN ZHU, JUN-FU ZHOU, WEI-YING SHAN, PEI-SU ZHOU, GUI-ZHONG TONG. Potential Oxidative Stress in the Bodies of Electric Arc Welding Operators:Effect of Photochemical Smog . Biomedical and Environmental Sciences, 2004, 17(4): 381-389.
[14]	P.DIPTI, B.YOGESH, A.K.KAIN, T.PAULINE, B.ANJU, M.SAIRAM, B.Singh, S.S.MONGIA, G.ILAVAZHAGAN DEVENDRA KUMAR, W.SELVAMURTHY. Lead Induced Oxidative Stress: Beneficial Effects of Kombucha Tea . Biomedical and Environmental Sciences, 2003, 16(3): 276-282.
[15]	JUN-FU ZHOU, YE-HUA ZHOU, Liang Zhang, Huai-Hong Chen, DONG CAI. 3,4-Methylenedioxymethamphetamine (MDMA) Abuse Markedly Inhibits Acetylcholinesterase Activity and Induces Severe Oxidative Damage and Liperoxidative Damage . Biomedical and Environmental Sciences, 2003, 16(1): 53-61.
[16]	JUN-FU ZHOU, WEI-WEI CHEN, GUI-ZHONG TONG. Ozone Emitted During Copying Process -A Potential Cause of Pathological Oxidative Stress and Potential Oxidative Damage in the Bodies of Operators . Biomedical and Environmental Sciences, 2003, 16(2): 95-104.
[17]	ZHOU JUN-FU, CHEN Jia-xi, SHEN Han-chao, CAI DONG. Abnormal Reactions of Free Radicals and Oxidative Damages in the Bodies of Patients With Chronic Glomerulonephritis . Biomedical and Environmental Sciences, 2002, 15(3): 233-244.
[18]	XIE Bo, ZHOU JUN-FU, LU Qun, LI Chong-jian, CHEN PENG. Oxidative Stress in Patients With Acute Coxsackie Virus Myocarditis . Biomedical and Environmental Sciences, 2002, 15(1): 48-57.
[19]	ZHOU JUN-FU, CHEN PENG. Studies on the Oxidative Stress in Alcohol Abusers in China . Biomedical and Environmental Sciences, 2001, 14(3): 180-188.
[20]	ZHOU JUN-FU, CHEN PENG, YANG JIN-LU, ZHU YOU-GEN, PENG Cheng-hong, WU Yu-lian. Oxidative Stress before and after Operation in Patients with Chronic Cholecystitis Containing Gallstone . Biomedical and Environmental Sciences, 2000, 13(4): 254-262.

点击查看大图

计量

文章访问数: 1522
HTML全文浏览量: 545
PDF下载量: 95
被引次数: 0

Antioxidant Effect of Sepia Ink Extract on Extrahepatic Cholestasis Induced by Bile Duct Ligation in Rats

doi: 10.3967/bes2015.082

刊出日期: 2015-08-20

关键词:

Bile duct ligation /

Hepatic fibrosis /

Oxidative stress /

Liver collagen percentage /

Histopathological examination

摘要: Objective The aim of our study was to assess the complications of hepatic fibrosis associated with bile duct ligation and the potential curative role of sepia ink extract in hepatic damage induced by bile duct ligation.
Methods Rattus norvegicus rats were divided into 3 groups: Sham-operated group, model rats that underwent common bile duct ligation (BDL), and BDL rats treated orally with sepia ink extract (200 mg/kg body weight) for 7, 14, and 28 d after BDL.
Results There was a significant reduction in hepatic enzymes, ALP, GGT, bilirubin levels, and oxidative stress in the BDL group after treatment with sepia ink extract. Collagen deposition reduced after sepia ink extract treatment as compared to BDL groups, suggesting that the liver was repaired. Histopathological examination of liver treated with sepia ink extract showed moderate degeneration in the hepatic architecture and mild degeneration in hepatocytes as compared to BDL groups.
Conclusion Sepia ink extract provides a curative effect and an antioxidant capacity on BDL rats and could ameliorate the complications of liver cholestasis.

English Abstract

Citation:

Hanan Saleh, Amel M Soliman, Ayman S Mohamed, Mohamed-Assem S Marie. Antioxidant Effect of Sepia Ink Extract on Extrahepatic Cholestasis Induced by Bile Duct Ligation in Rats[J]. Biomedical and Environmental Sciences, 2015, 28(8): 582-594. doi: 10.3967/bes2015.082