Fatma Guesmi; Hamida Beghalem; Amit K Tyagi; Manel Ben Ali; Ramla Ben Mouhoub; Houda Bellamine; Ahmed Landoulsi

doi:10.3967/bes2016.035

Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis

Fatma Guesmi, Hamida Beghalem, Amit K Tyagi, Manel Ben Ali, Ramla Ben Mouhoub, Houda Bellamine, Ahmed Landoulsi

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(4): 275-285

Fatma Guesmi, Hamida Beghalem, Amit K Tyagi, Manel Ben Ali, Ramla Ben Mouhoub, Houda Bellamine, Ahmed Landoulsi. Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 275-285. doi: 10.3967/bes2016.035

Citation:

Fatma Guesmi, Hamida Beghalem, Amit K Tyagi, Manel Ben Ali, Ramla Ben Mouhoub, Houda Bellamine, Ahmed Landoulsi. Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 275-285. doi: 10.3967/bes2016.035

doi: 10.3967/bes2016.035

Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis

Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte,Zarzouma, Bizerte 7021, Tunisia
Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, HoustonTX 77030, USA
Laboratory of Biomonitoring of the Environment LR01/ES14, Faculty of Sciences of Bizerte,Zarzouma, Bizerte 7021,Tunisia
Service of Anatomo- Pathology of Menzel Bourguiba, Bizerte 7050, Tunisia

摘要: ObjectiveWe evaluate the effects ofThymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats.
MethodsRats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg).
ResultsThe results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls.The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity andsignificant increase (P<0.05) in MDAconcentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2generated oxidative stress.These results were confirmed byhistological architecture examinations.
ConclusionH2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.
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Abstract: ObjectiveWe evaluate the effects ofThymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats.
MethodsRats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg).
ResultsThe results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls.The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity andsignificant increase (P<0.05) in MDAconcentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2generated oxidative stress.These results were confirmed byhistological architecture examinations.
ConclusionH2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.
- Testis /
- Hydrogen peroxide /
- TEO /
- Antioxidants /
- Infertility

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Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis

doi: 10.3967/bes2016.035

刊出日期: 2016-04-20

关键词:

Testis /

Hydrogen peroxide /

TEO /

Antioxidants /

Infertility

摘要: ObjectiveWe evaluate the effects ofThymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats.
MethodsRats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg).
ResultsThe results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls.The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity andsignificant increase (P<0.05) in MDAconcentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2generated oxidative stress.These results were confirmed byhistological architecture examinations.
ConclusionH2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.

English Abstract

Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis

Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte,Zarzouma, Bizerte 7021, Tunisia

Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, HoustonTX 77030, USA

Laboratory of Biomonitoring of the Environment LR01/ES14, Faculty of Sciences of Bizerte,Zarzouma, Bizerte 7021,Tunisia

Service of Anatomo- Pathology of Menzel Bourguiba, Bizerte 7050, Tunisia

Publish Date: 2016-04-20

Keywords:

Abstract: ObjectiveWe evaluate the effects ofThymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats.
MethodsRats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg).
ResultsThe results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls.The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity andsignificant increase (P<0.05) in MDAconcentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2generated oxidative stress.These results were confirmed byhistological architecture examinations.
ConclusionH2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.

Citation:

Fatma Guesmi, Hamida Beghalem, Amit K Tyagi, Manel Ben Ali, Ramla Ben Mouhoub, Houda Bellamine, Ahmed Landoulsi. Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 275-285. doi: 10.3967/bes2016.035