LI Hao; GUO ZhenYang; ZHANG Jian; TAO XiaoYan; ZHU WuYang; TANG Qing; LIU HongTu

doi:10.3967/bes2016.044

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(5): 340-346

LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu. Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China[J]. Biomedical and Environmental Sciences, 2016, 29(5): 340-346. doi: 10.3967/bes2016.044

Citation:

LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu. Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China[J]. Biomedical and Environmental Sciences, 2016, 29(5): 340-346. doi: 10.3967/bes2016.044

doi: 10.3967/bes2016.044

基金项目: the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX10004215)

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX10004215)

摘要: ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.
- /
- /
Abstract: ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.
- Rabies virus /
- Genome /
- Lineage

[1]	. One Health for Rabies Elimination: China Rabies Annual Conference in 2023 . Biomedical and Environmental Sciences, 2023, 36(6): 567-567. doi: 10.3967/bes2023.082
[2]	CHEN Sheng Lin, KANG Yu Tong, LIANG Yi He, QIU Xiao Tong, LI Zhen Jun. A Core Genome Multilocus Sequence Typing Scheme for Proteus mirabilis . Biomedical and Environmental Sciences, 2023, 36(4): 343-352. doi: 10.3967/bes2023.040
[3]	FENG Chang Zeng, ZHANG Ming, XU Dan Han, Guo Wei, SUN Hao, YANG Zhao Qing, MA Shao Hui. Complete Genome Analysis of A New Strain of Coxsackievirus A1 Associated with Severe HFMD in Yunnan, China . Biomedical and Environmental Sciences, 2022, 35(3): 248-253. doi: 10.3967/bes2022.034
[4]	SONG Qin Qin, LUO Xiao Nuan, SHI Bing Tian, LIU Mi, SONG Juan, XIA Dong, XIA Zhi Qiang, WANG Wen Jun, YAO Hai Lan, HAN Jun. Exploration of IRES Elements within the ORF of the Coxsackievirus B3 Genome . Biomedical and Environmental Sciences, 2022, 35(4): 322-333. doi: 10.3967/bes2022.043
[5]	ZHANG Jun Nan, MENG Ya Juan, BAI Yun Hua, LI Yu Feng, YANG Li Qing, SHI Nian Min, HAN Hui Xia, GAO Jian, ZHU Li Juan, LI Shu Ping, ZHANG Jing, ZHAO Qin Hua, WANG Xiu Qin, WEI Jing Shuang, REN Le Min, CAO Chen Hua, CHEN Chen, ZHAO Wei, LI Li. Rabies Virus Neutralizing Activity, Safety, and Immunogenicity of Recombinant Human Rabies Antibody Compared with Human Rabies Immunoglobulin in Healthy Adults . Biomedical and Environmental Sciences, 2022, 35(9): 782-791. doi: 10.3967/bes2022.103
[6]	JIANG Han Ji, CHEN Na, SHEN Zhi Qiang, YIN Jing, QIU Zhi Gang, MIAO Jing, YANG Zhong Wei, SHI Dan Yang, WANG Hua Ran, WANG Xin Wei, LI Jun Wen, YANG Dong, JIN Min. Inactivation of Poliovirus by Ozone and the Impact of Ozone on the Viral Genome . Biomedical and Environmental Sciences, 2019, 32(5): 324-333. doi: 10.3967/bes2019.044
[7]	MIAO Fa Ming, CHEN Teng, LIU Ye, ZHANG Shou Feng, ZHANG Fei, LI Nan, HU Rong Liang. Emerging New Phylogenetic Groups of Rabies Virus in Chinese Ferret Badgers . Biomedical and Environmental Sciences, 2018, 31(6): 479-482. doi: 10.3967/bes2018.064
[8]	LI Shao Li, SUN Hong Mei, ZHU Bao Li, LIU Fei, ZHAO Han Qing. Whole Genome Analysis Reveals New Insights into Macrolide Resistance in Mycoplasma pneumoniae . Biomedical and Environmental Sciences, 2017, 30(5): 343-350. doi: 10.3967/bes2017.045
[9]	MAO Li Li, XIAO Xin Hua, ZHANG Qian, ZHENG Jia, LI Wen Hui, YU Miao, ZHANG Hua Bing, PING Fan, XU Jian Ping, WANG Xiao Jing. DNA Methylation and Birth Weight:a Genome-wide Analysis . Biomedical and Environmental Sciences, 2017, 30(9): 667-670. doi: 10.3967/bes2017.088
[10]	ZOU Xiao Hui, CHEN Wen Bing, ZHAO Xiang, ZHU Wen Fei, YANG Lei, WANG Da Yan, SHU Yue Long. Evaluation ofA Single-reaction Method for Whole Genome Sequencing of Influenza A Virus using Next Generation Sequencing . Biomedical and Environmental Sciences, 2016, 29(1): 41-46. doi: 10.3967/bes2016.004
[11]	LI Xiao Ying, LI Ying, ZHANG Yao, KANG Wan Li, ZHAO Li Ping, DING Peng Ju, DAI Wen Tao, HUANG Hai Rong, HUANG Yan Feng, LI Wei Min. The Epidemiological Characteristics of Beijing Lineage Mycobacterium tuberculosis from a National Referral Center in China . Biomedical and Environmental Sciences, 2015, 28(7): 539-543. doi: 10.3967/bes2015.077
[12]	YANG Fang, CHEN Xiang Ding, TAN Li Jun, SHEN Jie, LI Ding You, ZHANG Fang, SHA Bao Yong, DENG Hong Wen. Genome Wide Association Study:Searching for Genes Underlying Body Mass Index in the Chinese . Biomedical and Environmental Sciences, 2014, 27(5): 360-370. doi: 10.3967/bes2014.061
[13]	YIN Jing Feng, DING Yu Lin, HUANG Ying, TAO Xiao Yan, LI Hao, YU Peng Cheng, SHEN Xin Xin, JIAO Wen Tao, LIANG GuoDong, TANG Qing, and WANG Feng Long. Comparative Analysis of the Pathogenic Mechanisms of Street Rabies Virus Strains with Different Virulence Levels . Biomedical and Environmental Sciences, 2014, 27(10): 749-762. doi: 10.3967/bes2014.034
[14]	LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China . Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046
[15]	XI Bo, MI Jie. Genome-wide Association Studies of Common Obesity: Now and Future . Biomedical and Environmental Sciences, 2013, 26(10): 787-791. doi: 10.3967/bes2013.001
[16]	HUANG Ying, TANG Qing, Simon RAYNER, GONG Kai, SONG Bo, LIANG Guo Dong. Pathogenicity of Rabies Viruses Isolated in China: Two Fixed Strains and a Street Strain . Biomedical and Environmental Sciences, 2013, 26(7): 552-561. doi: 10.3967/0895-3988.2013.07.006
[17]	ZHU Wu Yang, LIANG Guo Dong. Current Status of Canine Rabies in China . Biomedical and Environmental Sciences, 2012, 25(5): 602-605. doi: 10.3967/0895-3988.2012.05.016
[18]	LV Xin Jun, MA Xue Jun, WANG Li Hua, LI Hao, SHEN Xin Xin, YU Peng Cheng, TANG Qing, LIANG Guo Dong. Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein . Biomedical and Environmental Sciences, 2012, 25(1): 98-103. doi: 10.3967/0895-3988.2012.01.014
[19]	CAI Liang, TAO XiaoYan, LIU YunZhi, ZHANG Hong, GAO LiDong, HU ShiXiong, LIU FuQiang, LI Hao, SHEN XinXin, LIU JiaHui, WANG ShiQing, TANG Qing. Molecular Characteristics and Phylogenetic Analysis of N Gene of Human Derived Rabies Virus . Biomedical and Environmental Sciences, 2011, 24(4): 431-437. doi: 10.3967/0895-3988.2011.04.015
[20]	XIN-YU SHAO, RONG ZHANG, CHENG HU, CONG-RONG WANG, JING-YI LU, WEN QIN, HAO-YONG YU, YU-QIAN BAO, XING-BO CHENG, WEI-PING JIA. Precise Microdeletion Detection of Prader-Willi Syndrome with Array Comparative Genome Hybridization . Biomedical and Environmental Sciences, 2010, 23(3): 194-198.

点击查看大图

计量

文章访问数: 1114
HTML全文浏览量: 352
PDF下载量: 93
被引次数: 0

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

doi: 10.3967/bes2016.044

基金项目: the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX10004215)

刊出日期: 2016-05-20

关键词:

Rabies virus /

Genome /

Lineage

摘要: ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.

English Abstract

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX10004215)

Publish Date: 2016-05-20

Keywords:

Rabies virus /
Genome /
Lineage

Abstract: ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.

Citation:

LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu. Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China[J]. Biomedical and Environmental Sciences, 2016, 29(5): 340-346. doi: 10.3967/bes2016.044

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

Publish Under the Auspices of Chinese Center for Disease Control and Prevention

Distributed by Chinese Center for Disease Control and Prevention

Quick Links

Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net

doi: 10.3967/bes2016.044

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

计量

出版历程

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

doi: 10.3967/bes2016.044

English Abstract

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 102206, China

全文HTML

目录