2019 Vol. 32, No. 9

2019, 32(9): 1-1.
2019, 32(9): 2-2.
Original Article
The Mediating Effect of Body Dissatisfaction in Association between Obesity and Dietary Behavior Changes for Weight Loss in Chinese Children
GAO Huai Quan, WANG Bang Xuan, SUN Li Li, LI Ting, WU Lu, FU Lian Guo, MA Jun
2019, 32(9): 639-646. doi: 10.3967/bes2019.083
Objective The aim of this study was to analyze the mediating effect of body dissatisfaction in correlation between obesity and dietary behavior changes for weight loss (DBCWL). Methods A total of 680 primary and middle school students were included in this study. Their body height, weight, and waistline were effectively measured, and they were also evaluated to assess their body dissatisfaction, perception of dietary behaviors, and DBCWL. The correlation among these factors was analyzed using mediating effect models. Results The prevalence of overweight/obesity and abdominal obesity was significantly higher in males than in females (P < 0.05). Overweight/obesity, abdominal obesity, and body dissatisfaction significantly increased the risk for DBCWL (OR = 2.57, 2.77, and 1.95, respectively). Overweight/obesity and abdominal obesity significantly increased the risk for body dissatisfaction (OR = 6.00 and 4.70, respectively). Significant mediating effects of body dissatisfaction were observed in correlation between overweight/obesity and DBCWL and between abdominal obesity and DBCWL (OR = 2.20 and 1.92, respectively; P < 0.05), and the proportions of mediating effects among the total effects were 48.89% and 46.60%, respectively. Conclusion Body dissatisfaction might play an important mediating effect in association between DBCWL and obesity, which indicates that guiding children to correctly recognize their body might be more conducive than promoting obese children toward weight loss through dietary behavior changes.
Validity and Reliability of Chinese Physical Activity Questionnaire for Children Aged 10-17 Years
YANG Xi, JAGO Russell, ZHAI Yi, YANG Zhen Yu, WANG Yu Ying, SI Xiang, WANG Jun, GAO Jian Fen, CHEN Jing Ron, YU Ying Jie, ZHAO Wen Hua
2019, 32(9): 647-658. doi: 10.3967/bes2019.084
Objective This study is aimed to report the development, the reliability and validity of the Chinese Children Physical Activity Questionnaire (CCPAQ) which was designed for the assessment of physical activity pattern in young population in China. Methods The CCPAQ was administered for two times in 119 children (mean age 13.1 ± 2.4 years; boys 47%) to examine reliability by using intraclass correlation coefficients. Validity was determined in 106 participants by agreement with the CCPAQ measures and the objective method, the ActiGraph accelerometer. Data on physical activity pattern including time spent on different intensities and total physical activity, sedentary behavior as well as physical activity energy expenditure were used to assess the validity with Spearman’s correlation coefficient and the Bland-Altman plots. Results The reliability coefficient of the CCPAQ ranged from 0.63-0.93 (Intra-class correlation coefficient). Spearman’s correlation coefficient for validity of time spent on total physical activity and sedentary behavior were all 0.32 (P < 0.001), and for physical activity energy expenditure was 0.58 (P < 0.001). Time spent on moderate-to-vigorous physical activity and light physical activity showed a relatively low correlation with the accelerometer (rho = 0.20, P = 0.040; rho = 0.19, P = 0.054). Conclusion The CCPAQ appears to be a promising and feasible method to assess physical activity pattern in Chinese children.
Metabolomic Profiling Differences among Asthma, COPD, and Healthy Subjects: A LC-MS-based Metabolomic Analysis
LIANG Ying, GAI Xiao Yan, CHANG Chun, ZHANG Xu, WANG Juan, LI Ting Ting
2019, 32(9): 659-672. doi: 10.3967/bes2019.085
Objective Asthma and chronic obstructive pulmonary disease (COPD) feature different inflammatory and cellular profiles in the airways, indicating that the cellular metabolic pathways regulating these disorders are distinct. Methods We aimed to compare the serum metabolomic profiles among mild persistent asthmatic patients, individuals with stable COPD, and healthy subjects and to explore the potential metabolic biomarkers and pathways. The serum metabolomic profiles of 17 subjects with mild persistent asthma, 17 subjects with stable COPD, and 15 healthy subjects were determined by an untargeted metabolomic analysis utilizing liquid chromatography-mass spectrometry. A series of multivariate statistical analyses was subsequently used. Results Multivariate analysis indicated a distinct separation between the asthmatic patients and healthy controls in electrospray positive and negative ions modes, respectively. A total of 19 differential metabolites were identified. Similarly, a distinct separation between asthma and COPD subjects was detected in the two ions modes. A total of 16 differential metabolites were identified. Among the identified metabolites, the serum levels of hypoxanthine were markedly higher in asthmatic subjects compared with those in COPD or healthy subjects. Conclusions Patients with asthma present a unique serum metabolome, which can distinguish them from individuals with COPD and healthy subjects. Purine metabolism alteration may be distinct and involved in the pathogenesis of asthma.
Synthesis and Evaluation of a Novel Small-molecule Compound as an Anticancer Inhibitor of CD147
FU Zhi Guang, WANG Yan, WANG Shuang, SHAO Dan, TIAN Li, LI Yun Xia, JIANG Jian Li, CHEN Zhi Nan, WEN Ning
2019, 32(9): 673-686. doi: 10.3967/bes2019.086
Objective Cancer is a serious threat to human health. Despite extensive research on cancer treatment, there is a growing demand for new therapies. CD147 is widely involved in tumor development, but it is unclear whether cancer cell malignancy is affected by CD147 expression level. The first compound (AC-73) targeting CD147 could only act on advanced tumors and inhibit metastasis. Therefore, new compounds with better anticancer activity should be explored. Methods Wst-1 assays were used to confirm the effect of novel compounds on proliferation. Apoptosis tests were used to evaluate their proapoptotic capacity. A nude mouse model was used to demonstrate in vivo anticancer activity and safety of the compounds. Western blots were used to suggest a molecule mechanism. Results There is a positive correlation between CD147 expression and tumor cell proliferation. A new compound, HA-08, was synthesized and proved to be more active than AC-73. HA-08 could inhibit cancer cell viability and promote cancer cell apoptosis both in vitro and in vivo. HA-08 induces cancer apoptosis, mainly by disrupting the CD147-CD44 interaction and then down-regulating the JAK/STAT3/Bcl-2 signaling pathway. Conclusion Our results have clarified the tumor specificity of CD147 and its drug target characteristics. The biological profile of HA-08 suggests that this compound could be developed as a potential anticancer agent.
Effect of High Positive Acceleration (+Gz) Environment on Dental Implant Osseointegration:A Preliminary Animal Study
ZHU Xiao Ru, DENG Tian Zheng, PANG Jian Liang, LIU Bing, KE Jie
2019, 32(9): 687-698. doi: 10.3967/bes2019.087
Objective To observe the effect of high positive acceleration (+Gz) environment on dental implant osseointegration in a rabbit model and to investigate its mechanism. Methods Forty-eight New Zealand white rabbits were randomly divided into 6 groups. The rabbit’s mandibular incisors were extracted and 1 implant was placed in each socket immediately. After 1 week of rest, the rabbits were exposed to a high +Gz environment, 3 times a week. The rabbits were sacrificed at 3 weeks (2 weeks +Gz exposure), 5 weeks (4 weeks +Gz exposure), and 12 weeks (4 weeks +Gz exposure and 7 weeks normal environment) after surgery, respectively. Specimens were harvested for micro-CT scanning, histological analysis, and real-time polymerase chain reaction examination. Results Compared with those in the control group, the mRNA expression levels of bone morphogenetic protein-2 (BMP-2), osteopontin (OPN), and transforming growth factor-β1 (TGF-β1) were significantly lower (P < 0.05), while the mRNA expression level of receptor activator of nuclear factor κB ligand (RANKL) and the RANKL/osteoprotegerin (OPG) ratio were significantly higher (P < 0.05) at 3 weeks; values of bone volume fraction, trabecular number, bone-implant contact (BIC), and TGF-β1 and OPG mRNA expression levels were significantly lower (P < 0.05), and the value of trabecular separation, RANKL mRNA expression level and RANKL/OPG ratio were significantly higher (P < 0.05) at 5 weeks; and the value of BIC was still significantly lower (P < 0.05) at 12 weeks in the experimental group. Conclusion Early exposure to the high +Gz environment after implant surgery might have an adverse effect on osseointegration, and its mechanism could be related to the inhibition of osteoblast activity and promotion of osteoclast activity.
Letter to the Editor
Molecular Characterisation of Two Coxsackievirus B6 Strains from the Tibet Autonomous Region of China
SUN Qiang, HONG Mei, FENG Ning, ZHANG Yong
2019, 32(9): 699-703. doi: 10.3967/bes2019.088
Prevalence and Rates of New Diagnosis and Missed Diagnosis of Diabetes Mellitus among 35–74-year-old Residents in Urban Communities in Southwest China
CAO Xin, YANG Min, HUANG Xiao Bo, TAN Xue Ling, LIU Ya, HUO Nan, CHEN Ming Sheng, TANG Wei Wei, MA Xiao, XU Rong Hua
2019, 32(9): 704-709. doi: 10.3967/bes2019.089
Disparity Management Outcomes for Patients with Severe Mental Disorders in Sichuan Province: A Preliminary Evaluation from the National Surveillance Dataset
TANG Wei Wei, LIU Yuan Yuan, WANG Dan, WEN Hong, YANG Min
2019, 32(9): 710-714. doi: 10.3967/bes2019.090
Association of Meteorological Factors with Labial Adhesions in Children: A 7-year Retrospective Analysis with 9,467 Cases
ZHAO Tian Xin, LUO Qing, WANG Jun Ke, HAN Lin Dong, WEI Yue Xin, SUN Mang, LIU Bin, SHEN Lian Ju, LONG Chun Lan, YU Cheng Jun, LIN Tao, WU Sheng De, WEI Guang Hui
2019, 32(9): 715-718. doi: 10.3967/bes2019.091