LIU YuMei; ZHANG WenZhong; YONG Ling; ZHAO XiaoHong; JIA XuDong; LI Ning

doi:10.3967/0895-3988.2011.04.017

An Assessment of Androgenic/Anti-androgenic Effects of GH Transgenic Carp by Hershberger Assay

LIU YuMei, ZHANG WenZhong, YONG Ling, ZHAO XiaoHong, JIA XuDong, LI Ning

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(4): 445-449

LIU YuMei, ZHANG WenZhong, YONG Ling, ZHAO XiaoHong, JIA XuDong, LI Ning. An Assessment of Androgenic/Anti-androgenic Effects of GH Transgenic Carp by Hershberger Assay[J]. Biomedical and Environmental Sciences, 2011, 24(4): 445-449. doi: 10.3967/0895-3988.2011.04.017

Citation:

LIU YuMei, ZHANG WenZhong, YONG Ling, ZHAO XiaoHong, JIA XuDong, LI Ning. An Assessment of Androgenic/Anti-androgenic Effects of GH Transgenic Carp by Hershberger Assay[J]. Biomedical and Environmental Sciences, 2011, 24(4): 445-449. doi: 10.3967/0895-3988.2011.04.017

doi: 10.3967/0895-3988.2011.04.017

基金项目: the Major State Basic Research Development Program of China (973 Program)(2007CB109207)

An Assessment of Androgenic/Anti-androgenic Effects of GH Transgenic Carp by Hershberger Assay

College of Applied Arts and Sciences, Beijing Union University, Beijing 100191, China;National Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China
National Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China
College of Applied Arts and Sciences, Beijing Union University, Beijing 100191, China

Funds: the Major State Basic Research Development Program of China (973 Program)(2007CB109207)

摘要: Objective To evaluate the androgenic and anti-androgenic effects of GH (growth hormone) transgenic carp in male rats.Methods Hershberger assay was carried out in castrated male SD rats aged 4-5 weeks.Testosterone propionate (TP) (0.4 mg/kg BW) was administrated for a positive control,GH transgenic carp (3.0 g/kg BW)+TP (0.4 mg/kg BW),parental carp (3.0 g/kg BW) + TP (0.4 mg/kg BW),and flutamide (Flu) (3.0 g/kg BW) were used for negative controls,and vehicle was administered orally for a blank control.All groups were administrated for 10 consecutive days.At the end of the test,animals were anesthetized,then weights of accessory sex organ were measured.Serum testosterone (T),luteinizing hormone (LH),and Follicle-Stimulating Hormone (FSH) levels were detected.Results The weights ratios of the accessory sex organs and body weights showed no significant differences between the solvent control and the GH transgenic carp-treated groups.Serum concentrations of FSH,LH,and T of the rats treated with GH transgenic carp + TP showed no significant changes,compared with those treated with TP only.Conclusion GH transgenic carp does not have any androgenic agonist or antagonist properties in vivo screening tests.
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Abstract: Objective To evaluate the androgenic and anti-androgenic effects of GH (growth hormone) transgenic carp in male rats.Methods Hershberger assay was carried out in castrated male SD rats aged 4-5 weeks.Testosterone propionate (TP) (0.4 mg/kg BW) was administrated for a positive control,GH transgenic carp (3.0 g/kg BW)+TP (0.4 mg/kg BW),parental carp (3.0 g/kg BW) + TP (0.4 mg/kg BW),and flutamide (Flu) (3.0 g/kg BW) were used for negative controls,and vehicle was administered orally for a blank control.All groups were administrated for 10 consecutive days.At the end of the test,animals were anesthetized,then weights of accessory sex organ were measured.Serum testosterone (T),luteinizing hormone (LH),and Follicle-Stimulating Hormone (FSH) levels were detected.Results The weights ratios of the accessory sex organs and body weights showed no significant differences between the solvent control and the GH transgenic carp-treated groups.Serum concentrations of FSH,LH,and T of the rats treated with GH transgenic carp + TP showed no significant changes,compared with those treated with TP only.Conclusion GH transgenic carp does not have any androgenic agonist or antagonist properties in vivo screening tests.
- GH gene transgenic carp /
- Hershberger assay /
- Androgenic properties

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An Assessment of Androgenic/Anti-androgenic Effects of GH Transgenic Carp by Hershberger Assay

doi: 10.3967/0895-3988.2011.04.017

基金项目: the Major State Basic Research Development Program of China (973 Program)(2007CB109207)

刊出日期: 2011-08-20

关键词:

GH gene transgenic carp /

Hershberger assay /

Androgenic properties

摘要: Objective To evaluate the androgenic and anti-androgenic effects of GH (growth hormone) transgenic carp in male rats.Methods Hershberger assay was carried out in castrated male SD rats aged 4-5 weeks.Testosterone propionate (TP) (0.4 mg/kg BW) was administrated for a positive control,GH transgenic carp (3.0 g/kg BW)+TP (0.4 mg/kg BW),parental carp (3.0 g/kg BW) + TP (0.4 mg/kg BW),and flutamide (Flu) (3.0 g/kg BW) were used for negative controls,and vehicle was administered orally for a blank control.All groups were administrated for 10 consecutive days.At the end of the test,animals were anesthetized,then weights of accessory sex organ were measured.Serum testosterone (T),luteinizing hormone (LH),and Follicle-Stimulating Hormone (FSH) levels were detected.Results The weights ratios of the accessory sex organs and body weights showed no significant differences between the solvent control and the GH transgenic carp-treated groups.Serum concentrations of FSH,LH,and T of the rats treated with GH transgenic carp + TP showed no significant changes,compared with those treated with TP only.Conclusion GH transgenic carp does not have any androgenic agonist or antagonist properties in vivo screening tests.

English Abstract

An Assessment of Androgenic/Anti-androgenic Effects of GH Transgenic Carp by Hershberger Assay

College of Applied Arts and Sciences, Beijing Union University, Beijing 100191, China;National Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China

National Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China

College of Applied Arts and Sciences, Beijing Union University, Beijing 100191, China

Funds: the Major State Basic Research Development Program of China (973 Program)(2007CB109207)

Publish Date: 2011-08-20

Keywords:

Abstract: Objective To evaluate the androgenic and anti-androgenic effects of GH (growth hormone) transgenic carp in male rats.Methods Hershberger assay was carried out in castrated male SD rats aged 4-5 weeks.Testosterone propionate (TP) (0.4 mg/kg BW) was administrated for a positive control,GH transgenic carp (3.0 g/kg BW)+TP (0.4 mg/kg BW),parental carp (3.0 g/kg BW) + TP (0.4 mg/kg BW),and flutamide (Flu) (3.0 g/kg BW) were used for negative controls,and vehicle was administered orally for a blank control.All groups were administrated for 10 consecutive days.At the end of the test,animals were anesthetized,then weights of accessory sex organ were measured.Serum testosterone (T),luteinizing hormone (LH),and Follicle-Stimulating Hormone (FSH) levels were detected.Results The weights ratios of the accessory sex organs and body weights showed no significant differences between the solvent control and the GH transgenic carp-treated groups.Serum concentrations of FSH,LH,and T of the rats treated with GH transgenic carp + TP showed no significant changes,compared with those treated with TP only.Conclusion GH transgenic carp does not have any androgenic agonist or antagonist properties in vivo screening tests.

Citation: