GONG Han Shi; TIAN Chan; ZHANG Bao Yurn; WANG Zhao Yun; XIE Wu Ling; JING Yuan Yuan; GAO Chen; JIANG Hui Ying; SHI Qi; LIU Yong; DONG Xiao Ping

doi:10.3967/0895-3988.2011.06.004

doi: 10.3967/0895-3988.2011.06.004

基金项目: Chinese National Natural Science Foundation(30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101)%the SKLID Development Grant(2008SKLID102%2008SKLID202)

Establishment of Hamster- and Human-PRNP Transgenic Mice

School of Medicine, Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, China; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
School of Medicine, Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, China

Funds: Chinese National Natural Science Foundation(30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101)%the SKLID Development Grant(2008SKLID102%2008SKLID202)

摘要: Objective To create transgenic mice expressing hamster- and human-PRNP as a model for understanding the physiological function and pathology of prion protein (PrP),as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies (TSEs).Methods Hamster and human-PRNP transgenic mice were established by conventional methods.The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR.PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR,real-time RT-PCR,and western blot analysis.Histological analyses of transgenic mice were performed by hematoxylin and eosin (H & E) staining and immunohistochemical (IHC) methods.Results Integrated PRNP copy number in various mouse lines was 53 (Tg-haPrP1),18 (Tg-huPrP1),3 (Tg-huPrP2),and 16 (Tg-huPrP5),respectively.Exogenous PrPs were expressed at both the transcriptional and translational level.Histological assays did not detect any abnormalities in brain or other organs.Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines.These four transgenic mouse lines provide ideal models for additional research.
- /
- /
- /
Abstract: Objective To create transgenic mice expressing hamster- and human-PRNP as a model for understanding the physiological function and pathology of prion protein (PrP),as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies (TSEs).Methods Hamster and human-PRNP transgenic mice were established by conventional methods.The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR.PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR,real-time RT-PCR,and western blot analysis.Histological analyses of transgenic mice were performed by hematoxylin and eosin (H & E) staining and immunohistochemical (IHC) methods.Results Integrated PRNP copy number in various mouse lines was 53 (Tg-haPrP1),18 (Tg-huPrP1),3 (Tg-huPrP2),and 16 (Tg-huPrP5),respectively.Exogenous PrPs were expressed at both the transcriptional and translational level.Histological assays did not detect any abnormalities in brain or other organs.Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines.These four transgenic mouse lines provide ideal models for additional research.
- PrP /
- PRNP /
- Transgenic mice /
- Copy number

[1]	MENG Li Ying, TAO Wen Fu, LI Jing, ZHU Min, ZHONG De Bin, ZHOU Jing, QIN Xue, WEI Rong Guo. Effects of Bisphenol A and Its Substitute, Bisphenol F, on the Gut Microbiota in Mice . Biomedical and Environmental Sciences, 2024, 37(1): 19-30. doi: 10.3967/bes2024.003
[2]	YAN Zhao Fan, GU Zhi Guang, FAN Ya Hui, LI Xin Ling, NIU Ze Ming, DUAN Xiao Ran, Mallah Ali Manthar, ZHANG Qiao, YANG Yong Li, YAO Wu, WANG Wei. Benchmark Dose Assessment for Coke Oven Emissions-Induced Mitochondrial DNA Copy Number Damage Effects . Biomedical and Environmental Sciences, 2023, 36(6): 490-500. doi: 10.3967/bes2023.060
[3]	LI Yan Yan, DING Wen Hao, BAI Yi Chun, WANG Lei, WANG Yong Bin. Estimating the Effects of the COVID-19 Outbreak on the Decreasing Number of Acquired Immune Deficiency Syndrome Cases and Epidemiological Trends in China . Biomedical and Environmental Sciences, 2022, 35(2): 141-145. doi: 10.3967/bes2022.019
[4]	ZHANG Yi, AN Ming Xing, GONG Chen, LI Yang Yang, WANG Yu Tong, LIN Meng, LI Rong, TIAN Chan. Single-copy Loss of Rho Guanine Nucleotide Exchange Factor 10 (arhgef10) Causes Locomotor Abnormalities in Zebrafish Larvae . Biomedical and Environmental Sciences, 2022, 35(1): 35-44. doi: 10.3967/bes2022.005
[5]	ZHANG Wen Ping, ZHANG Yi Fan, ZHANG Ying Ying, HAN Zhi Chao, GAO Yuan Yuan, GUO Jian Yong, SHI Xiu Jing, HU Xiao Qin, MU Li Na, ZHOU Yun, LEI Li Jian. Peripheral Blood Mitochondrial DNA Copy Number and Hypertension Combined with Albuminuria in Chinese Coal Miners . Biomedical and Environmental Sciences, 2021, 34(7): 567-571. doi: 10.3967/bes2021.078
[6]	QU Shui Ling, WANG Ai Ling, PAN Xiao Ping, WANG XiaoYan, LUO Hui Ming, ZHANG Tong. Calculating the Number of Pregnant Women Receiving Standardized Services for PMTCT of HIV in Liangshan Prefecture, Based on the Equivalent Method . Biomedical and Environmental Sciences, 2020, 33(10): 745-749. doi: 10.3967/bes2020.099
[7]	YANG Xue Hua, WU Yue Zhang, XIAO Kang, GAO Li Ping, CHEN Dong Dong, DONG Xiao Ping, SHI Qi. Preparation of PrP-specific Polyclonal Antibody via Immunization of PRNP-knockout Mice with Recombinant Human PrP Protein . Biomedical and Environmental Sciences, 2020, 33(7): 493-501. doi: 10.3967/bes2020.066
[8]	GUO Zhen Yong, ZHAO Wei Jie, ZHENG Mei Qin, LIU Shuo, YAN Chen Xia, LI Peng, XU Shao Fa. Activities of Biapenem against Mycobacterium tuberculosis in Macrophages and Mice . Biomedical and Environmental Sciences, 2019, 32(4): 235-241. doi: 10.3967/bes2019.033
[9]	WANG Yuan, LI Yu Ling, MENG Fan Zhu, HOU Bao Lian, ZHUANG Chuan Ning, XIONG Shu Han, REN Shu Ping. Effects of Omethoate on Liver Insulin Signaling in Mice . Biomedical and Environmental Sciences, 2018, 31(8): 627-631. doi: 10.3967/bes2018.086
[10]	HU Yuan, LI Bo Qing, JIN Da Zhi, HE Li Hua, TAO Xiao Xia, ZHANG Jian Zhong. Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in Acinetobacter pittii and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme . Biomedical and Environmental Sciences, 2015, 28(12): 855-863. doi: 10.3967/bes2015.119
[11]	FANG Jin, LIANG Chun Lai, JIA Xu Dong, LI Ning. Immunotoxicity of Acrylamide in Female BALB/c Mice . Biomedical and Environmental Sciences, 2014, 27(6): 401-409. doi: 10.3967/bes2014.069
[12]	ZHOU Xin, HOU Xue Xia, GENG Zhen, ZHAO Rui, WAN Kang Lin, HAO Qin. Establishment of Multiple Locus Variable-number Tandem Repeat Analysis Assay for Genotyping of Borrelia burgdorferi sensu lato Detected in China . Biomedical and Environmental Sciences, 2014, 27(9): 665-675. doi: 10.3967/bes2014.102
[13]	SONG Miao, TANG Qing, Rayner Simon, TAO Xiao Yan, SHEN Xin Xin, LIANG Guo Dong. Factors Influencing the Number of Rabies Cases in Children in China . Biomedical and Environmental Sciences, 2014, 27(8): 627-632. doi: 10.3967/bes2014.095
[14]	ZHANG Xiao Ai, BAI Xue Mei, YE Chang Yun, REN Zhi Hong, XU Jian Guo. Establishment and Comparison of Pulsed-field Gel Electrophoresis, Multiple-locus Variable Number Tandem Repeat Analysis and Automated Ribotyping Methods for Subtyping of Citrobacter Strains . Biomedical and Environmental Sciences, 2012, 25(6): 653-662. doi: 10.3967/0895-3988.2012.06.007
[15]	LIU YuMei, ZHANG WenZhong, YONG Ling, ZHAO XiaoHong, JIA XuDong, LI Ning. An Assessment of Androgenic/Anti-androgenic Effects of GH Transgenic Carp by Hershberger Assay . Biomedical and Environmental Sciences, 2011, 24(4): 445-449. doi: 10.3967/0895-3988.2011.04.017
[16]	. The First Chinese Case of Creutzfeldt-Jakob Disease with Mutation of E200K in PRNP . Biomedical and Environmental Sciences, 2010, 23(2): 158-160.
[17]	KANG-CHU LI, SHI-RONG MA, GUI-RONG DING, YAO GUO, GUO-ZHEN GUO. Effects of Electromagnetic Pulse on Bone Metabolism of Mice in vivo . Biomedical and Environmental Sciences, 2009, 22(6): 518-521.
[18]	XIN-LI XIAO, HUI-YING JIANG, JIN ZHANG, JUN HAN, KAI NIE, XIAO-BO ZHOU, YIN-XIA HUANG, LAN CHEN, WEI ZHOU, BAO-YUN ZHANG, YONG LIU, XIAO-PING DONG. Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP . Biomedical and Environmental Sciences, 2005, 18(4): 273-280.
[19]	Hong WAN, HIROSHI ISHIHARA. Expression of JunB Induced by X-rays in Mice . Biomedical and Environmental Sciences, 2004, 17(3): 327-332.
[20]	JIAN-MEI GAO, CHEN GAO, JUN HAN, Xiao-Bo Zhou, XIN-LI XIAO, Jin Zhang, LAN CHEN, BAO-YUN ZHANG, TAO HONG, XIAO-PING DONG. Dynamic Analyses of PrP and PrPsc in Brain Tissues of Golden Hamsters Infected With Scraple Strain 263K Revealed Various PrP Forms . Biomedical and Environmental Sciences, 2004, 17(1): 8-20.

点击查看大图

计量

文章访问数: 1171
HTML全文浏览量: 277
PDF下载量: 41
被引次数: 0

doi: 10.3967/0895-3988.2011.06.004

Establishment of Hamster- and Human-PRNP Transgenic Mice

计量

Establishment of Hamster- and Human-PRNP Transgenic Mice

doi: 10.3967/0895-3988.2011.06.004

English Abstract

Establishment of Hamster- and Human-PRNP Transgenic Mice

全文HTML

目录

doi: 10.3967/0895-3988.2011.06.004

Establishment of Hamster- and Human-PRNP Transgenic Mice

计量

出版历程

Establishment of Hamster- and Human-PRNP Transgenic Mice

doi: 10.3967/0895-3988.2011.06.004

English Abstract

Establishment of Hamster- and Human-PRNP Transgenic Mice

全文HTML

目录