Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy

HAN YanLing,  LI Yuan,  SONG Juan,  WANG Ying,  SHI Qi,  CHEN Cao,  ZHANG BaoYun,  GUO Yan,  LI ChaoPing,  HAN Jun,  DONG XiaoPing

downloadPDF
文章导航 > Biomedical and Environmental Sciences  > 2011 >  24(5): 523-529
HAN YanLing, LI Yuan, SONG Juan, WANG Ying, SHI Qi, CHEN Cao, ZHANG BaoYun, GUO Yan, LI ChaoPing, HAN Jun, DONG XiaoPing. Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy[J]. Biomedical and Environmental Sciences, 2011, 24(5): 523-529. doi: 10.3967/0895-3988.2011.05.011
Citation: HAN YanLing, LI Yuan, SONG Juan, WANG Ying, SHI Qi, CHEN Cao, ZHANG BaoYun, GUO Yan, LI ChaoPing, HAN Jun, DONG XiaoPing. Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy[J]. Biomedical and Environmental Sciences, 2011, 24(5): 523-529. doi: 10.3967/0895-3988.2011.05.011
shu

doi: 10.3967/0895-3988.2011.05.011
  • , 
  • , 
  • , 
  • , 
  • , 
  • , 
  • , 
  • , 
  • , 
  • , 
基金项目: Chinese National Natural Science Foundation(Grants 30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101%2008ZX10004-001%%2008ZX10004-002)%the SKLID Development Grant(2008SKLID102%2008SKLI0202)

Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy

  • State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Medical School,Anhui University of Science and Technology, Huainan 231001, China

  • State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

  • Medical School,Anhui University of Science and Technology, Huainan 231001, China

Funds: Chinese National Natural Science Foundation(Grants 30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101%2008ZX10004-001%%2008ZX10004-002)%the SKLID Development Grant(2008SKLID102%2008SKLI0202)

WeChat 点击查看大图
计量
  • 文章访问数:  1199
  • HTML全文浏览量:  270
  • PDF下载量:  43
  • 被引次数: 0
出版历程
  • 刊出日期:  2011-10-20

Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy

doi: 10.3967/0895-3988.2011.05.011
    基金项目:  Chinese National Natural Science Foundation(Grants 30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101%2008ZX10004-001%%2008ZX10004-002)%the SKLID Development Grant(2008SKLID102%2008SKLI0202)
  • 刊出日期: 2011-10-20

摘要: Objective To break immune tolerance to prion (PrP) proteins using DNA vaccines.Methods Four different human prion DNA vaccine candidates were constructed based on the pcDNA3.1 vector:PrP-WT expressing wild-type PrP,Ubiq-PrP expressing PrP fused to ubiquitin,PrP-LII expressing PrP fused to the lysosomal integral membrane protein type II lysosome-targeting signal,and PrP-ER expressing PrP locating the ER.Using a prime-boost strategy,three-doses of DNA vaccine were injected intramuscularly into Balb/c mice,followed by two doses of PrP protein.Two weeks after the last immunization,sera and spleens were collected and PrP-specific humoral and cellular immune responses evaluated by ELISA and ELISPOT tests.Results Higher levels of serum PrP antibodies were detected in mice vaccinated using the strategy of DNA priming followed by protein boosting.Of these,WT-PrP,Ubiq-PrP,and PrP-LII induced significantly higher humoral responses.ELISPOT tests showed markedly increased numbers of IFN-γ-secreting T cells in mice vaccinated using the strategy of DNA priming followed by protein boosting after stimulation with recombinant PrP23-90 and PrP23-231.PrP-ER induced the strongest T-cell response.Conclusion Prion vaccines can break tolerance to PrP proteins and induce PrP-specific humoral and cellular immune responses.

English Abstract

HAN YanLing, LI Yuan, SONG Juan, WANG Ying, SHI Qi, CHEN Cao, ZHANG BaoYun, GUO Yan, LI ChaoPing, HAN Jun, DONG XiaoPing. Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy[J]. Biomedical and Environmental Sciences, 2011, 24(5): 523-529. doi: 10.3967/0895-3988.2011.05.011
Citation: HAN YanLing, LI Yuan, SONG Juan, WANG Ying, SHI Qi, CHEN Cao, ZHANG BaoYun, GUO Yan, LI ChaoPing, HAN Jun, DONG XiaoPing. Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy[J]. Biomedical and Environmental Sciences, 2011, 24(5): 523-529. doi: 10.3967/0895-3988.2011.05.011
shu

    全文HTML

目录

    /

    返回文章
    返回

    Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

    Publish Under the Auspices of Chinese Center for Disease Control and Prevention

    Distributed by Chinese Center for Disease Control and Prevention

    All Rights Reserved by Chinese Center for Disease Control and Prevention 京ICP备11024750-4

    Quick Links

    Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net 百度统计