Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts

HAO Chang Fu LI Xiao Fang YAO Wu

HAO Chang Fu, LI Xiao Fang, YAO Wu. Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts[J]. Biomedical and Environmental Sciences, 2013, 26(12): 979-985. doi: 10.3967/bes2013.033
Citation: HAO Chang Fu, LI Xiao Fang, YAO Wu. Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts[J]. Biomedical and Environmental Sciences, 2013, 26(12): 979-985. doi: 10.3967/bes2013.033

doi: 10.3967/bes2013.033
基金项目: the Research Fund from the National Natural Science Foundation of China (#81102109)

Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts

Funds: the Research Fund from the National Natural Science Foundation of China (#81102109)
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  • 刊出日期:  2013-12-20

Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts

doi: 10.3967/bes2013.033
    基金项目:  the Research Fund from the National Natural Science Foundation of China (#81102109)

摘要: Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts
Methods Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels ofα-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.
Results The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.
Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.

English Abstract

HAO Chang Fu, LI Xiao Fang, YAO Wu. Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts[J]. Biomedical and Environmental Sciences, 2013, 26(12): 979-985. doi: 10.3967/bes2013.033
Citation: HAO Chang Fu, LI Xiao Fang, YAO Wu. Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts[J]. Biomedical and Environmental Sciences, 2013, 26(12): 979-985. doi: 10.3967/bes2013.033

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