Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus

GAO Hui GONG Xiao Cheng CHEN Ze Dong XU Xiao Shuang ZHANG Quan XU Xiang Ming

GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082
Citation: GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082

doi: 10.3967/bes2014.082
基金项目: ThisworkwassupportedbyNatural Science Foundationof Jiangsu Province(BK20130445)%National Natural Science foundation of China(31302042)

Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus

Funds: ThisworkwassupportedbyNatural Science Foundationof Jiangsu Province(BK20130445)%National Natural Science foundation of China(31302042)
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  • 刊出日期:  2014-07-20

Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus

doi: 10.3967/bes2014.082
    基金项目:  ThisworkwassupportedbyNatural Science Foundationof Jiangsu Province(BK20130445)%National Natural Science foundation of China(31302042)

摘要: ObjectiveInactivated Sendai virus particle [hemagglutinating virus of Japan envelope (HVJ-E)] has a potential oncolytic effect due to its ability to induce apoptosis in tumor cells. However, the molecular mechanism of apoptosis induction in cancer cellsmediated by HVJ-E has not been fully elucidated.This paper aims to investigate the underlying mechanism of apoptosis induction by HVJ-E in prostate cancer cells (PC3). MethodsPC3 cells were treated with HVJ-E at various MOI, and theninterferon-β (IFN-β) production, and the cell viability and apoptosis were detected by ELISA, MTT-based assay and flow cytometry, respectively. Next, the roles of Jak-Stat, MAPK and Akt pathways played in HVJ-E-induced apoptosis in PC3 cells were analyzed by immunoblot assay. To further evaluate the cytotoxic effect of HVJ-E on PC3 cells, HVJ-E was intratumorally injected into prostate cancers on BALB/c-nude mice, and the tumor volume was monitored for 36 days. ResultsHVJ-E induced IFN-β production and activatedJak-Stat signaling pathway, which resulted in the activation of caspase-8, caspase-3, and PARP in PC3 prostate cancer cells post HVJ-E treatment. Furthermore, we observed for the first time that p38 and Jnk MAPKs in PC3 cells contributed to HVJ-E-induced apoptosis. In addition,intratumoralHVJ-E treatmentdisplayed a directinhibitoryeffect in anin vivo BALB/cnude mouseprostate cancermodel. ConclusionOur findingshaveprovided novel insights into the underlying mechanismsby whichHVJ-E induces apoptosisin tumor cells.

English Abstract

GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082
Citation: GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082

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