Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways

XIANG Ruo Lan MEIMei SU Yun Chao LI Li WANG Jin Yu WU Li Ling

XIANG Ruo Lan, MEIMei, SU Yun Chao, LI Li, WANG Jin Yu, WU Li Ling. Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways[J]. Biomedical and Environmental Sciences, 2015, 28(3): 169-177. doi: 10.3967/bes2015.023
Citation: XIANG Ruo Lan, MEIMei, SU Yun Chao, LI Li, WANG Jin Yu, WU Li Ling. Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways[J]. Biomedical and Environmental Sciences, 2015, 28(3): 169-177. doi: 10.3967/bes2015.023

doi: 10.3967/bes2015.023
基金项目: This study was supported by grants from the NationalNatural Science Foundation of China(.81100763 and 81270158)%Research Fund for Doctoral Programs of Higher Education(20120001110009)

Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways

Funds: This study was supported by grants from the NationalNatural Science Foundation of China(.81100763 and 81270158)%Research Fund for Doctoral Programs of Higher Education(20120001110009)
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  • 刊出日期:  2015-03-20

Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways

doi: 10.3967/bes2015.023
    基金项目:  This study was supported by grants from the NationalNatural Science Foundation of China(.81100763 and 81270158)%Research Fund for Doctoral Programs of Higher Education(20120001110009)

摘要: ObjectiveInterferon-γ (IFN-γ) plays an important role in apoptosis and was shown to increase the risk of diabetes.Visfatin, an adipokine, has anti-diabetic, anti-tumor, and regulating inflammatory properties. In this study we investigated the effect of visfatin on IFN-γ-induced apoptosis in rat pancreatic β-cells. MethodsThe RINm5F (rat insulinoma cell line) cells exposed to IFN-γ were treated with or without visfatin. The viability and apoptosis of the cells were assessed by using MTT and flow cytometry. The expressionsof mRNA and protein were detected by using real-time PCR and western blot analysis. ResultsThe exposure of RINm5F cells to IFN-γ for 48 h led to increased apoptosis percentage of the cells. Visfatin pretreatment significantly increased the cellviability and reduced the cell apoptosis induced by IFN-γ. IFN-γ-induced increase in expression of p53 mRNA and cytochrome c protein, decrease in mRNA and protein levels of anti-apoptotic protein Bcl-2 were attenuated by visfatin pretreatment. Visfatin alsoincreasedAMPK and ERK1/2phosphorylation and the anti-apoptotic action of visfatin was attenuated by the AMPK and ERK1/2 inhibitor. ConclusionThese results suggested that visfatin protected pancreatic islet cells against IFN-γ-induced apoptosis via mitochondria-dependent apoptotic pathway. The anti-apoptotic action of visfatin is mediated by activation of AMPK and ERK1/2 signaling molecules.

English Abstract

XIANG Ruo Lan, MEIMei, SU Yun Chao, LI Li, WANG Jin Yu, WU Li Ling. Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways[J]. Biomedical and Environmental Sciences, 2015, 28(3): 169-177. doi: 10.3967/bes2015.023
Citation: XIANG Ruo Lan, MEIMei, SU Yun Chao, LI Li, WANG Jin Yu, WU Li Ling. Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways[J]. Biomedical and Environmental Sciences, 2015, 28(3): 169-177. doi: 10.3967/bes2015.023

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