2021 Vol. 34, No. 10

2021, 34(10): 0-0.
2021, 34(10): 1-2.
Original Article
Association of Visit-to-Visit Variabilities in Metabolic Factors with Chronic Kidney Disease in Chinese Adults Living in Shanghai
LI Ling, WANG Fei, XU Min, LU Jie Li, ZHAO Zhi Yun, LI Mian, WANG Tian Ge, WANG Shuang Yuan, BI Yu Fang, XU Yu, CAI Wei Min, NING Guang
2021, 34(10): 761-772. doi: 10.3967/bes2021.106
  Objective  This study aimed to examine the association of visit-to-visit variabilities in metabolic factors with chronic kidney disease (CKD) in Shanghai community residents.  Methods  We used data from a cohort study of community residents who participated in three examinations in 2008, 2009, and 2013, respectively. Fasting plasma glucose (FPG) level, blood pressure (BP), and lipid levels were determined in 2,109 participants at all three visits, and CKD was evaluated between the second and the third visits. Visit-to-visit variabilities in metabolic factors were described by coefficients of variation (CV) at three visits. A variability score was calculated by adding the numbers of metabolic factors with a high variability defined as the highest quartile of CV. CKD was defined as the estimated glomerular filtration rate < 60 mL/min per 1.73 m2 or urinary albumin-to-creatinine ratio ≥ 30 mg/g.  Results  A total of 200 (9.5%) participants had CKD at the third visit. Compared with the lowest quartile of CV, the highest quartile was associated with a 70% increased risk of CKD for FPG [odds ratio, OR = 1.70; 95% confidence interval (CI) 1.06–2.72], 62% for systolic BP (OR = 1.62, 95% CI 1.04–2.50), and 85% for low-density lipoprotein cholesterol (OR = 1.85, 95% CI 1.23–2.80). Furthermore, the risk of CKD increased significantly with an increasing variability score. Compared with participants with score 0, participants with scores of 1, 2, and 3 were associated with 58% (OR = 1.58, 95% CI 1.08–2.32), 121% (OR = 2.21, 95% CI 1.40–3.49), and 548% (OR = 6.48, 95% CI 3.18–13.21) higher risks of CKD, respectively.  Conclusion  The visit-to-visit variabilities in metabolic factors were significantly associated with the risks of CKD in Shanghai community residents.
A Ten-year Retrospective Study of Invasive Candidiasis in a Tertiary Hospital in Beijing
YANG Zhi Hui, SONG Ying Gai, LI Ruo Yu
2021, 34(10): 773-788. doi: 10.3967/bes2021.107
  Objective  This study aimed to evaluate the epidemiological, clinical and mycological characteristics of invasive candidiasis (IC) in China.   Methods  A ten-year retrospective study including 183 IC episodes was conducted in a tertiary hospital in Beijing, China.   Results  The overall incidence of IC from 2010–2019 was 0.261 episodes per 1,000 discharges. Candidemia (71.0%) was the major infective pattern; 70.3% of the patients tested positive for Candida spp. colonization before IC and the median time to develop an invasive infection after colonization was 13.5 days (interquartile range: 4.5–37.0 days). Candida albicans (45.8%) was the most prevalent species, followed by Candida parapsilosis (19.5%), Candida glabrata (14.2%) and Candida tropicalis (13.7%). C. non-albicans IC was more common in patients with severe anemia (P = 0.018), long-term hospitalization (P = 0.015), hematologic malignancies (P = 0.002), continuous administration of broad-spectrum antibiotics (P < 0.001) and mechanical ventilation (P = 0.012). In vitro resistance testing showed that 11.0% of the Candida isolates were resistant/non-wild type (non-WT) to fluconazole, followed by voriconazole (9.6%), micafungin (3.8%), and caspofungin (2.9%). Fluconazole was the most commonly used drug to initiate antifungal therapy both before and after the proven diagnosis (52.6% and 54.6%, respectively). The 30-day and 90-day all-cause mortality rates were 24.5% and 32.7%, respectively.   Conclusion  The incidence of IC has declined in the recent five years. C. non-albicans contributed to more than half of the IC cases. Fluconazole can be used as first-line therapy if resistant strains are not prevalent. Prospective, multi-center surveillance of the clinical and mycological characteristics of IC is required.
Ionizing Radiation-Induced RPL23a Reduction Regulates Apoptosis via RPL11-MDM2-p53 Pathway in Mouse Spermatogonia
HE Yu Xuan, GUO Yi Xiao, ZHANG Yong, HU Jun Jie, DONG Wei Tao, DU Xiang Hong, ZHAO Xing Xu
2021, 34(10): 789-802. doi: 10.3967/bes2021.108
  Objective  The expression patterns of ribosomal large subunit protein 23a (RPL23a) in mouse testes and GC-1 cells were analyzed to investigate the potential relationship between RPL23a expression and spermatogonia apoptosis upon exposure to X-ray.   Methods  Male mice and GC-1 cells were irradiated with X-ray, terminal dUTP nick end-labelling (TUNEL) was performed to detect apoptotic spermatogonia in vivo. Apoptotic rate and cell cycle phase of GC-1 cells were analyzed with flow cytometry. Protein interactions were detected by Immunoprecipitation and protein localization as studied by immunofluorescence. Immunoblotting and real-time PCR were applied to analyze to protein and gene expression.   Results  Ionizing radiation (IR) increased spermatogonia apoptosis, the expression of RPL11, MDM2 and p53, and decreased RPL23a expression in mice spermatogonia in vivo and in vitro. RPL23a knockdown weakened the interaction between RPL23a and RPL11, leading to p53 accumulation. Moreover, knockdown and IR decreased RPL23a that induces spermatogonia apoptosis via RPL23a-RPL11-MDM2-p53 pathway in GC-1 cells.   Conclusion  These results suggested that IR reduced RPL23a expression, leading to weakened the RPL23a-RPL11 interactions, which may have activated p53, resulting in spermatogonia apoptosis. These results provide insights into environmental and clinical risks of radiotherapy following exposure to IR in male fertility. The graphical abstract was available in the web of www.besjournal.com.
Predicted 10-year Cardiovascular Disease Risk and Its Association with Sleep Duration among Adults in Beijing-Tianjin-Hebei Region, China
WANG Yu Xue, ZHANG Li, LI Chun Jun, QI Xin, FAN Ya Qi, HE Jiang Shan, GUO Pei, HU Jia Lin, CHEN Shuo, NIU Yu Jie, LIU Feng, ZHANG Rong, LI Qiang, MA Shi Tao, ZHANG Mian Zhi, HONG Cheng Lin, ZHANG Min Ying
2021, 34(10): 803-813. doi: 10.3967/bes2021.109
  Objective  The study aims to predict 10-year cardiovascular disease (CVD) risk and explore its association with sleep duration among Chinese urban adults.  Methods  We analyzed part of the baseline data of a cohort that recruited adults for health screening by cluster sampling. The simplified Pittsburgh Sleep Quality Index (PSQI) and Framingham 10-year risk score (FRS) were used to measure sleep duration and CVD risk. Demographic characteristics, personal history of chronic diseases, lifestyle factors were collected using a questionnaire. Height, weight, total cholesterol (TC), and high-density lipoprotein cholesterol (HDL-C) were also measured. Multiple logistic regression models were performed to explore the association of sleep duration with the predicted CVD risk.  Results  We included 31, 135 participants (median age 44 years, 53.02% males) free of CVD, cerebral stroke, and not taking lipid-lowering agents. Overall, 14.05%, and 25.55% of participants were at medium and high predicted CVD risk, respectively. Short sleep was independently associated with increased odds of medium to high risk of predicted 10-year CVD among males (OR = 1.10; 95% CI: 1.01–1.19) and increased odds of medium to high and high risk of predicted 10-year CVD among females (OR = 1.23; 95% CI: 1.08–1.40; OR = 1.27; 95% CI: 1.11–1.44). In contrast, long sleep had no association with cardiovascular risk.  Conclusion  A substantial number of adults free of CVD were at high 10-year CVD risk. Short sleep was associated with increased odds of predicted CVD risk.
Letter to the Editor
The Combined Effect of Dyslipidemia on the Incidence of Type 2 Diabetes: A Prospective Cohort Study in Northwest of China
WANG Min Zhen, DAI Tian, ZHENG Shan, YU Cheng, XIA Miao, YANG Hong Yan, ZHANG De Sheng, YIN Chun, JIN Ya Fei, CHENG Ning, BAI Ya Na
2021, 34(10): 814-818. doi: 10.3967/bes2021.110
Obesity rather than Metabolic Syndrome is a Risk Factor for Subclinical Hypothyroidism and Thyroid Autoimmunity
YAO Jin Yin, LIU Peng, ZHANG Wei, WANG Ke Wei, LYU Chun Peng, ZHANG Zhi Wei, CHEN Xiang Lan, CHEN Yi, WANG Xue Song, DING Yong Xia, MA Li Jun, WANG Jing, SUN Dian Jun
2021, 34(10): 819-823. doi: 10.3967/bes2021.111
Molecular Spectrum of α- and β-Thalassemia among Young Individuals of Marriageable Age in Guangdong Province, China
ZHOU Bing Yi, WANG Yong Xia, XU Shan Shan, GU Heng, LI Ming Zhen
2021, 34(10): 824-829. doi: 10.3967/bes2021.112
Thalassemia is a group of genetically heterogeneous diseases characterized by hemolytic anemia. To investigate molecular characteristics of α- and β-thalassemia among young individuals of marriageable age in Guangdong Province, 24,788 subjects with suspected thalassemia were genetically tested for α- and β-thalassemia by Gap-PCR and reverse dot blot during 2018–2019. For suspected rare thalassemia cases, DNA sequencing was performed to identify rare and unknown thalassemia gene mutations. A total of 14,346 thalassemia carriers were detected, including 7,556 cases of α-thalassemia with 25 genotypes and 8 α-gene mutations identified, 5,860 cases of β-thalassemia with 18 genotypes and 18 β-gene mutations identified, and 930 cases of compound α/β-thalassemia. Among them, the frequency of --SEA deletion was the highest in α-thalassemia (66.01%), followed by -α3.7 (17.98%) and -α4.2 (8.22%), and the frequency of CD41-42 (-TCTT) mutation was the highest in β-thalassemia (38.38%), followed by IVS-II-654 (C > T) (25.67%), -28 (A > G) (15.76%), and CD17 (10.01%). In addition, 5 rare mutations (--THAI and HKαα, CD113, -90, and CD56) were found in the study population. Our results revealed molecular epidemiological background of α- and β-thalassemia in Guangdong Province, which can support optimization of thalassemia prevention and control strategies. We demonstrated that thalassemia is heterogeneous with significant geographical differences and population specificity.
Cerebrospinal Fluids from Patients with Five Common Genetic Prion Diseases in China Display Distinct Reactivities in the RT-QuIC Assays
HU Chao, CHEN Cao, CHEN Jia, XIAO Kang, ZHOU Wei, XIA Ying, YANG Wei, WANG Lin, SHI Qi, DONG Xiao Ping
2021, 34(10): 830-833. doi: 10.3967/bes2021.113
Isolation and Characterization of Vagococcus fluvialis from Bats
QIN Tian, JIANG Lu Xi, REN Hong Yu, JIA Xue Yang, NIE Xu Dong, LI Yi Nan
2021, 34(10): 834-837. doi: 10.3967/bes2021.114
Relationship between TERT Polymorphism and Telomere Length in Workers Exposed to Omethoate
CHENG Shuai, LIU Bin, GUO Zhi Feng, DUAN Xiao Ran, LIU Su Xiang, LI Lei, YAO Wu, YANG Yong Li, WANG Wei
2021, 34(10): 838-841. doi: 10.3967/bes2021.115
Deep Learning in Chest Radiography: Detection of Pneumoconiosis
LI Xiao, LIU Chao Fei, GUAN Li, WEI Shu, YANG Xin, LI Shu Qiang
2021, 34(10): 842-845. doi: 10.3967/bes2021.116