ZHANG Ya Mei; ZHOU Yan; QIU Lian Bo; DING Gui Rong; PANG Xiao Feng

doi:10.3967/0895-3988.2012.02.011

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(2): 197-202

ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng. Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change[J]. Biomedical and Environmental Sciences, 2012, 25(2): 197-202. doi: 10.3967/0895-3988.2012.02.011

Citation:

ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng. Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change[J]. Biomedical and Environmental Sciences, 2012, 25(2): 197-202. doi: 10.3967/0895-3988.2012.02.011

doi: 10.3967/0895-3988.2012.02.011

基金项目: The National Key Basic Research and Development Plan of China(212011CB503701)

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China
Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shanxi, China

Funds: The National Key Basic Research and Development Plan of China(212011CB503701)

摘要: Objective To investigate the expression of occludin,ZO-1,MMP-2,and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.Methods Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n=8).After exposure to PEMF at 0.5,1,3,6,and 12 h,BBB permeability was measured by Evans-Blue extravasation.The expression of occludin,ZO-1,MMP-2,and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting.MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.Results Compared with the sham group,PEMF exposure led to increased permeability of the BBB to EB,which was prolonged after exposure.BBB permeability became progressively more severe,and recovered at 6 h.The gene and protein expression of occludin and ZO-1 were significantly decreased,while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF.All levels of expression recovered 12 h following PEMF.Conclusion Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.
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Abstract: Objective To investigate the expression of occludin,ZO-1,MMP-2,and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.Methods Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n=8).After exposure to PEMF at 0.5,1,3,6,and 12 h,BBB permeability was measured by Evans-Blue extravasation.The expression of occludin,ZO-1,MMP-2,and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting.MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.Results Compared with the sham group,PEMF exposure led to increased permeability of the BBB to EB,which was prolonged after exposure.BBB permeability became progressively more severe,and recovered at 6 h.The gene and protein expression of occludin and ZO-1 were significantly decreased,while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF.All levels of expression recovered 12 h following PEMF.Conclusion Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.
- Pulse electromagnetic field /
- Blood-brain barrier /
- Cerebral microvasculature

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Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

doi: 10.3967/0895-3988.2012.02.011

基金项目: The National Key Basic Research and Development Plan of China(212011CB503701)

刊出日期: 2012-04-20

关键词:

Pulse electromagnetic field /

Blood-brain barrier /

Cerebral microvasculature

摘要: Objective To investigate the expression of occludin,ZO-1,MMP-2,and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.Methods Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n=8).After exposure to PEMF at 0.5,1,3,6,and 12 h,BBB permeability was measured by Evans-Blue extravasation.The expression of occludin,ZO-1,MMP-2,and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting.MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.Results Compared with the sham group,PEMF exposure led to increased permeability of the BBB to EB,which was prolonged after exposure.BBB permeability became progressively more severe,and recovered at 6 h.The gene and protein expression of occludin and ZO-1 were significantly decreased,while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF.All levels of expression recovered 12 h following PEMF.Conclusion Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.

English Abstract

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shanxi, China

Funds: The National Key Basic Research and Development Plan of China(212011CB503701)

Publish Date: 2012-04-20

Keywords:

Abstract: Objective To investigate the expression of occludin,ZO-1,MMP-2,and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.Methods Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n=8).After exposure to PEMF at 0.5,1,3,6,and 12 h,BBB permeability was measured by Evans-Blue extravasation.The expression of occludin,ZO-1,MMP-2,and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting.MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.Results Compared with the sham group,PEMF exposure led to increased permeability of the BBB to EB,which was prolonged after exposure.BBB permeability became progressively more severe,and recovered at 6 h.The gene and protein expression of occludin and ZO-1 were significantly decreased,while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF.All levels of expression recovered 12 h following PEMF.Conclusion Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.

Citation:

ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng. Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change[J]. Biomedical and Environmental Sciences, 2012, 25(2): 197-202. doi: 10.3967/0895-3988.2012.02.011

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doi: 10.3967/0895-3988.2012.02.011

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

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Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

doi: 10.3967/0895-3988.2012.02.011

English Abstract

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shanxi, China

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School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shanxi, China