LIU Jun Chen; ZHOU Chen Hui; ZHANG Xue; CHEN Yan; XU Bi Lian; CUI Liao; XU Dao Hua

doi:10.3967/bes2014.113

Effect of 1,25-Dihydroxyvitamin D3 on Regulatory T Cells in Ovariectomized Mice

LIU Jun Chen, ZHOU Chen Hui, ZHANG Xue, CHEN Yan, XU Bi Lian, CUI Liao, XU Dao Hua

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(10): 779-785

LIU Jun Chen, ZHOU Chen Hui, ZHANG Xue, CHEN Yan, XU Bi Lian, CUI Liao, XU Dao Hua. Effect of 1,25-Dihydroxyvitamin D3 on Regulatory T Cells in Ovariectomized Mice[J]. Biomedical and Environmental Sciences, 2014, 27(10): 779-785. doi: 10.3967/bes2014.113

Citation:

LIU Jun Chen, ZHOU Chen Hui, ZHANG Xue, CHEN Yan, XU Bi Lian, CUI Liao, XU Dao Hua. Effect of 1,25-Dihydroxyvitamin D3 on Regulatory T Cells in Ovariectomized Mice[J]. Biomedical and Environmental Sciences, 2014, 27(10): 779-785. doi: 10.3967/bes2014.113

doi: 10.3967/bes2014.113

基金项目: This study was supported by Natural Science Foundation of Guangdong Province(10152402301000000)%Science and Technology Planning Project of Dongguan(2011108102019)%Science & Technology Innovation Fund of Guangdong Medical College(STIF201104)

Effect of 1,25-Dihydroxyvitamin D3 on Regulatory T Cells in Ovariectomized Mice

Department of Pharmacology, Guangdong Medical College, Dongguan 523808,Guangdong,China

Funds: This study was supported by Natural Science Foundation of Guangdong Province(10152402301000000)%Science and Technology Planning Project of Dongguan(2011108102019)%Science & Technology Innovation Fund of Guangdong Medical College(STIF201104)

摘要: ObjectiveTo investigate thecorrelation betweenregulatory T(Treg) cellsand postmenopausal osteoporosis andthe antiosteoporotic effect of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] in relation to Treg cells. MethodsFifty femaleBALB/c mice were randomly divided into five groups: the basal control (BAS), Sham,ovariectomy (OVX),OVX+diethylstilbestrol (OVX+DES), andOVX+1,25(OH)2D3.Tibias were harvested andprocessed with decalcification for quantitative bone histomorphometry.Femurs were stained by immunohistochemistry to detectFoxp3 protein expression.Spleens wereused to detect Treg andFoxp3 gene expressionby flow cytometry andquantitative RT-PCR, respectively. ResultsIn comparison withthe Sham group,a significant decrease was found inthe OVX group in such indices as trabecular bone volume/total tissue area (BV/TV), trabecularnumber (Tb.N) and trabecular thickness (Tb.Th).1,25(OH)2D3andDESpartlyprevented the decrease in BV/TV, Tb.N, Tb.Th inOVX mice.Treg cell number, Foxp3 mRNA expression in spleen andFoxp3 protein expression in femur significantly decreasedinthe OVX-treated group compared withthose in thesham group.1,25(OH)2D3 andDES significantlyincreased Treg cell number andFoxp3 expression.Treg cellsand Foxp3 gene expression were related to bonehistomorphometricparameters. ConclusionThedecrease inTreg cell numbersis relevant to the postmenopausal osteoporosis. The antiosteoporosis of1,25(OH)2D3 is related to regulatory T cells.
- /
- /
- /
Abstract: ObjectiveTo investigate thecorrelation betweenregulatory T(Treg) cellsand postmenopausal osteoporosis andthe antiosteoporotic effect of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] in relation to Treg cells. MethodsFifty femaleBALB/c mice were randomly divided into five groups: the basal control (BAS), Sham,ovariectomy (OVX),OVX+diethylstilbestrol (OVX+DES), andOVX+1,25(OH)2D3.Tibias were harvested andprocessed with decalcification for quantitative bone histomorphometry.Femurs were stained by immunohistochemistry to detectFoxp3 protein expression.Spleens wereused to detect Treg andFoxp3 gene expressionby flow cytometry andquantitative RT-PCR, respectively. ResultsIn comparison withthe Sham group,a significant decrease was found inthe OVX group in such indices as trabecular bone volume/total tissue area (BV/TV), trabecularnumber (Tb.N) and trabecular thickness (Tb.Th).1,25(OH)2D3andDESpartlyprevented the decrease in BV/TV, Tb.N, Tb.Th inOVX mice.Treg cell number, Foxp3 mRNA expression in spleen andFoxp3 protein expression in femur significantly decreasedinthe OVX-treated group compared withthose in thesham group.1,25(OH)2D3 andDES significantlyincreased Treg cell number andFoxp3 expression.Treg cellsand Foxp3 gene expression were related to bonehistomorphometricparameters. ConclusionThedecrease inTreg cell numbersis relevant to the postmenopausal osteoporosis. The antiosteoporosis of1,25(OH)2D3 is related to regulatory T cells.
- 1,25-dihydroxyvitamin D3 /
- Osteoporosis /
- Regulatory T cells /
- Ovariectomy

[1]	JIAO Ning, JI Wan Sheng, ZHANG Biao, SHANG Yu Kui, ZHANG Yu Chen, YU Wei Qun, JIN Hai Long, LI Chao, ZHANG Cheng Ying, YAN Cheng, YUE Wen, ZHANG Qing. Overexpression of Protein Phosphatase 2 Regulatory Subunit B"Alpha Promotes Glycolysis by Regulating Hexokinase 1 in Hepatocellular Carcinoma . Biomedical and Environmental Sciences, 2022, 35(7): 622-632. doi: 10.3967/bes2022.082
[2]	CHEN Peng, ZHUANG Chuan Ning, CUI Jia Jing, WANG Ping Wei, LIU Dong Ge, YAN Shu Qi, ZHOU Li Ting, REN Shu Ping. Effects of Combination of 1,25(OH)₂D₃ and TLR-4 Inhibitor on the Damage to HaCaT Cells Caused by UVB Irradiation . Biomedical and Environmental Sciences, 2022, 35(11): 1051-1062. doi: 10.3967/bes2022.133
[3]	ZHAO Hong, QIU Ting Ting, LIU Mei Qing, CHEN Lin Xi. SENP2: A Novel Regulatory Mechanism of Brown Adipocyte Differentiation . Biomedical and Environmental Sciences, 2020, 33(11): 872-876. doi: 10.3967/bes2020.119
[4]	WU Chang Yue, YIN Kai Zhi, ZHANG Yan, JIAO Man, ZHAO Xin Yuan, WU Qi Yun. 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin Promotes Proliferation of Astrocyte Cells via the Akt/STAT3/Cyclin D1 Pathway . Biomedical and Environmental Sciences, 2019, 32(4): 281-290. doi: 10.3967/bes2019.038
[5]	CHEN Xue Lan, ZHANG Bin, TANG Li, JIAO Hai Tao, XU Heng Yi, XU Feng, XU Hong, WEI Hua, XIONG Yong Hua. Expression and Characterization of ArgR, An Arginine Regulatory Protein in Corynebacterium crenatum . Biomedical and Environmental Sciences, 2014, 27(6): 436-443. doi: 10.3967/bes2014.072
[6]	DONG Juan Cong, CHENG Guang Hui, SHAN Yu Xing, WU Ning, SHAO Ming Long, LI Peng Wu, JIN Shun Zi. Role of PLC-PIP2 and cAMP-PKA Signal Pathways in Radiation-induced Immune-suppressing Effect . Biomedical and Environmental Sciences, 2014, 27(1): 27-34. doi: 10.3967/bes2014.005
[7]	GE Jiao, LIU Yan, LI Qiang, GUO Xia, GU Ling, MA Zhi Gui, ZHU Yi Ping. Resveratrol Induces Apoptosis and Autophagy in T-cell Acute Lymphoblastic Leukemia Cells by Inhibiting Akt/mTOR and Activating p38-MAPK . Biomedical and Environmental Sciences, 2013, 26(11): 902-911. doi: 10.3967/bes2013.019
[8]	ZHU Yan Feng, HU Jia Zhe, ZHAO Pin Nan, LIU Lin Xi, LI Yun. All-transRetinoic Acid Regulates Th1/Th2 Balance in CD4+ T cells When GATA-3 is Deficient . Biomedical and Environmental Sciences, 2013, 26(9): 774-777. doi: 10.3967/0895-3988.2013.09.010
[9]	LIN Si En, HUANG Jian Ping, WU Ling Zhi, WU Tie, CUI Liao. Prevention of Osteopenia and Dyslipidemia in Rats after Ovariectomy with Combined Aspirin and Low-dose Diethylstilbestrol . Biomedical and Environmental Sciences, 2013, 26(4): 249-257. doi: 10.3967/0895-3988.2013.04.003
[10]	DENG Min, WANG Yi Xiang, James F. GRIFFITH, LU Gang, Anil T AHUJA, POON Wai S. Characteristics of Rat Lumbar Vertebral Body Bone Mineral Density and Differential Segmental Responses to Sex Hormone Deficiency: a Clinical Multidetector Computed Tomography Study . Biomedical and Environmental Sciences, 2012, 25(6): 607-613. doi: 10.3967/0895-3988.2012.06.001
[11]	LING WANG, YAN XIE, LI-JING ZHU, TING-TING CHANG, YAN-QING MAO, JIE LI. An Association between Immunosenescence and CD4+CD25+ Regulatory T Cells: A Systematic Review . Biomedical and Environmental Sciences, 2010, 23(4): 327-332.
[12]	ZHE WANG, HONG-WEI LIU, KUN-XUE HONG, ZU-JIANG YU, JIAN-PING CHEN, YU-HUA RUAN, QUAN-CHENG KAN, YI-MING SHAO. Complete Human Immunodeficiency Virus-1 Specific T Lymphocyte Response to Chinese Human Immunodeficiency Virus-1 B/C Chronic Infectors . Biomedical and Environmental Sciences, 2009, 22(6): 522-528.
[13]	SU-ZHEN JIANG, ZHI-YONG GAO, TONG LI, YA-JUAN LI, XIANG-MEI CHEN, LING WANG, FENG-MIN LU, HUI ZHUANG. T3098C and T53C Mutations of HBV Genotype C Is Associated with HBV Infection Progress1 . Biomedical and Environmental Sciences, 2009, 22(6): 511-517.
[14]	SHAO-LING WANG, YING EI, YING WEN, YAN-FENG CHEN, LI-XIN NA, SONG-TAO LI, CHANG-HAO SUN. Curcumin, a Potential Inhibitor of Up-regulation of TNF-alpha and IL-6 Induced by Palmitate in 3T3-L1 Adipocytes through NF-kappaB and JNK Pathway . Biomedical and Environmental Sciences, 2009, 22(1): 32-39.
[15]	MIN YANG, CHANG-CHUN QIU, WEI CHEN, LING-LING XU, MIAO YU, HONG-DING XIANG. Identification of a Regulatory Single Nucleotide Polymorphism in the Adiponectin (APM1) Gene Associated with Type2 Diabetes in Han Nationality . Biomedical and Environmental Sciences, 2008, 21(6): 454-459.
[16]	MING-MING JIA, KUN-XUE HONG, JIAN-PING CHEN, HONG-WEI LIU, SHA LIU, XIAO-QING ZHANG, HONG-JING ZHAO, YI-MING SHAO. CTL Responses to Regulatory Proteins Tat and Rev in HIV-1 B'/C Virus-Infected Individuals . Biomedical and Environmental Sciences, 2008, 21(4): 314-318.
[17]	LONG-GUO YUAN, HONG-BIN DENG, LI-HUI CHEN, DIAN-DONG LI, QI-YANG HE. Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells . Biomedical and Environmental Sciences, 2008, 21(3): 212-217.
[18]	XIAO-LIN NA, JUNKO EZAKI, FUMIE SUGIYAMA, HONG-BIN CUI, YOSHIKO ISHIMI. Isoflavone Regulates Lipid Metabolism via Expression of Related Genes in OVX Rats Fed on a High-fat Diet . Biomedical and Environmental Sciences, 2008, 21(5): 357-364.
[19]	YAN-QUN LIU, YI-KAI ZHOU. In vitro Cytotoxicity of TCDD on SPC-A1 Cells . Biomedical and Environmental Sciences, 2006, 19(1): 21-26.
[20]	MA Qing-wen, LIN Guo-fang, CHEN JIG-ANG, SHEN JIAN-HUA. Polymorphism of Glutathione S-transferase T1, M1 and P1 Genes in a Shanghai Population: Patients With Occupational or Non-occupational Bladder Cancer . Biomedical and Environmental Sciences, 2002, 15(3): 253-260.

点击查看大图

计量

文章访问数: 1252
HTML全文浏览量: 342
PDF下载量: 69
被引次数: 0

Effect of 1,25-Dihydroxyvitamin D3 on Regulatory T Cells in Ovariectomized Mice

doi: 10.3967/bes2014.113

刊出日期: 2014-10-20

关键词:

1,25-dihydroxyvitamin D3 /

Osteoporosis /

Regulatory T cells /

Ovariectomy

摘要: ObjectiveTo investigate thecorrelation betweenregulatory T(Treg) cellsand postmenopausal osteoporosis andthe antiosteoporotic effect of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] in relation to Treg cells. MethodsFifty femaleBALB/c mice were randomly divided into five groups: the basal control (BAS), Sham,ovariectomy (OVX),OVX+diethylstilbestrol (OVX+DES), andOVX+1,25(OH)2D3.Tibias were harvested andprocessed with decalcification for quantitative bone histomorphometry.Femurs were stained by immunohistochemistry to detectFoxp3 protein expression.Spleens wereused to detect Treg andFoxp3 gene expressionby flow cytometry andquantitative RT-PCR, respectively. ResultsIn comparison withthe Sham group,a significant decrease was found inthe OVX group in such indices as trabecular bone volume/total tissue area (BV/TV), trabecularnumber (Tb.N) and trabecular thickness (Tb.Th).1,25(OH)2D3andDESpartlyprevented the decrease in BV/TV, Tb.N, Tb.Th inOVX mice.Treg cell number, Foxp3 mRNA expression in spleen andFoxp3 protein expression in femur significantly decreasedinthe OVX-treated group compared withthose in thesham group.1,25(OH)2D3 andDES significantlyincreased Treg cell number andFoxp3 expression.Treg cellsand Foxp3 gene expression were related to bonehistomorphometricparameters. ConclusionThedecrease inTreg cell numbersis relevant to the postmenopausal osteoporosis. The antiosteoporosis of1,25(OH)2D3 is related to regulatory T cells.

English Abstract

Effect of 1,25-Dihydroxyvitamin D3 on Regulatory T Cells in Ovariectomized Mice

Department of Pharmacology, Guangdong Medical College, Dongguan 523808,Guangdong,China

Publish Date: 2014-10-20

Keywords:

Abstract: ObjectiveTo investigate thecorrelation betweenregulatory T(Treg) cellsand postmenopausal osteoporosis andthe antiosteoporotic effect of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] in relation to Treg cells. MethodsFifty femaleBALB/c mice were randomly divided into five groups: the basal control (BAS), Sham,ovariectomy (OVX),OVX+diethylstilbestrol (OVX+DES), andOVX+1,25(OH)2D3.Tibias were harvested andprocessed with decalcification for quantitative bone histomorphometry.Femurs were stained by immunohistochemistry to detectFoxp3 protein expression.Spleens wereused to detect Treg andFoxp3 gene expressionby flow cytometry andquantitative RT-PCR, respectively. ResultsIn comparison withthe Sham group,a significant decrease was found inthe OVX group in such indices as trabecular bone volume/total tissue area (BV/TV), trabecularnumber (Tb.N) and trabecular thickness (Tb.Th).1,25(OH)2D3andDESpartlyprevented the decrease in BV/TV, Tb.N, Tb.Th inOVX mice.Treg cell number, Foxp3 mRNA expression in spleen andFoxp3 protein expression in femur significantly decreasedinthe OVX-treated group compared withthose in thesham group.1,25(OH)2D3 andDES significantlyincreased Treg cell number andFoxp3 expression.Treg cellsand Foxp3 gene expression were related to bonehistomorphometricparameters. ConclusionThedecrease inTreg cell numbersis relevant to the postmenopausal osteoporosis. The antiosteoporosis of1,25(OH)2D3 is related to regulatory T cells.

Citation: