2022 Vol. 35, No. 2

2022, 35(2): 0-0.
2022, 35(2): 1-2.
Original Article
Caprylic Acid Improves Lipid Metabolism, Suppresses the Inflammatory Response and Activates the ABCA1/p-JAK2/p-STAT3 Signaling Pathway in C57BL/6J Mice and RAW264.7 Cells
ZHANG Xin Sheng, ZHANG Peng, LIU Ying Hua, XU Qing, ZHANG Yong, LI Hui Zi, LIU Lu, LIU Yu Meng, YANG Xue Yan, XUE Chang Yong
2022, 35(2): 95-106. doi: 10.3967/bes2022.014
  Objective  This study aimed to investigate the effects of caprylic acid (C8:0) on lipid metabolism and inflammation, and examine the mechanisms underlying these effects in mice and cells.   Methods  Fifty-six 6-week-old male C57BL/6J mice were randomly allocated to four groups fed a high-fat diet (HFD) without or with 2% C8:0, palmitic acid (C16:0) or eicosapentaenoic acid (EPA). RAW246.7 cells were randomly divided into five groups: normal, lipopolysaccharide (LPS), LPS+C8:0, LPS+EPA and LPS+cAMP. The serum lipid profiles, inflammatory biomolecules, and ABCA1 and JAK2/STAT3 mRNA and protein expression were measured.   Results  C8:0 decreased TC and LDL-C, and increased the HDL-C/LDL-C ratio after injection of LPS. Without LPS, it decreased TC in mice (P < 0.05). Moreover, C8:0 decreased the inflammatory response after LPS treatment in both mice and cells (P < 0.05). Mechanistic investigations in C57BL/6J mouse aortas after injection of LPS indicated that C8:0 resulted in higher ABCA1 and JAK2/STAT3 expression than that with HFD, C16:0 and EPA, and resulted in lower TNF-α, NF-κB mRNA expression than that with HFD (P < 0.05). In RAW 264.7 cells, C8:0 resulted in lower expression of pNF-κBP65 than that in the LPS group, and higher protein expression of ABCA1, p-JAK2 and p-STAT3 than that in the LPS and LPS+cAMP groups (P < 0.05).   Conclusion  Our studies demonstrated that C8:0 may play an important role in lipid metabolism and the inflammatory response, and the mechanism may be associated with ABCA1 and the p-JAK2/p-STAT3 signaling pathway.
Analysis of Radial Peripapillary Capillary Density in Patients with Bietti Crystalline Dystrophy by Optical Coherence Tomography Angiography
ZHANG Sheng Juan, WANG Li Fei, XIAO Zhe, LIU Zhi Qiang, XING Chen, LI Qian, SUN Hui Jing, YANG Zan Zhang, LYU Li Na, PENG Xiao Yan
2022, 35(2): 107-114. doi: 10.3967/bes2022.015
  Objective  We wanted to investigate the radial peripapillary capillary (RPC) network in patients with Bietti crystalline dystrophy (BCD).   Methods  We compared RPC densities in the disk and different peripapillary regions, obtained using optical coherence tomography angiography in 22 patients with BCD (37 eyes) and 22 healthy subjects (37 eyes). The BCD group was then divided into Stage 2 and Stage 3 subgroups based on Yuzawa staging, comparing the RPC densities of the two.   Results  The disk area RPC density was 38.8% ± 6.3% in the BCD group and 49.2% ± 6.1% in the control group (P < 0.001), and peripapillary region RPC density was significantly lower in the BCD group than in the control group (49.1% ± 4.7% and 54.1% ± 3.0%, respectively, P < 0.001). There were no significant RPC density differences between the tempo quadrant and inside disk of Stages 2 and 3 subgroups; the other areas showed a significantly lower RPC density in Stage 3 than in Stage 2 BCD.   Conclusion  The BCD group RPC density was significantly lower than the control group. The reduction of RPC density in the tempo quadrant occurred mainly in the Stage 1 BCD. In contrast, the reduction of RPC density in superior, inferior, and nasal quadrants occurred mainly in Stage 2.
Polymorphisms of the Vitamin D Receptor Gene and Sex-Differential Associations with Lipid Profiles in Chinese Han Adults
CHEN Yan Mei, XU Ping, WANG Zhou Tian, ZHU Yu Mei, GONG Chun Mei, HUANG Chang Hua, LIU Xiao Li, ZHOU Ji Chang
2022, 35(2): 115-125. doi: 10.3967/bes2022.016
  Objective  To explore the association of single nucleotide polymorphisms (SNPs) of the vitamin D receptor gene (VDR) with circulating lipids considering gender differences.  Methods  Of the Han Chinese adults recruited from a health examination center for inclusion in the study, the circulating lipids, 25-hydroxyvitamin D (25OHD), and other parameters were measured. The VDR SNPs of Cdx2 (rs11568820), Fok1 (rs2228570), Apa1 (rs7975232), and Taq1 (rs731236) were genotyped with a qPCR test using blood DNA samples, and their associations with lipids were analyzed using logistic regression.  Results  In the female participants (n = 236 with dyslipidemia and 888 without dyslipidemia), multiple genotype models of Fok1 indicated a positive correlation of B (not A) alleles with LDLC level (P < 0.05). In the male participants (n = 299 with dyslipidemia and 564 without dyslipidemia), the recessive model of Cdx2 and the additive and recessive models of Fok1 differed (P < 0.05) between the HDLC-classified subgroups, respectively, and Fok1 BB and Cdx2 TT presented interactions with 25OHD in the negative associations with HDLC (P < 0.05).  Conclusion  In the Chinese Han adults included in the study, the Fok1 B-allele of VDR was associated with higher LDLC in females, and the Fok1 B-allele and the Cdx2 T-allele of VDR were associated with lower HDLC in males. The interaction of VD and Fok1 BB or Cdx2 TT in males synergistically decreased HDLC levels.
Association of C-reactive Protein with Cardiovascular Outcomes: A Mendelian Randomization Study in the Japanese Population
CAO Ming Yang, LIU Di, ZHANG Xiao Yu, TIAN Qiu Yue, ZHANG Qun, WANG You Xin
2022, 35(2): 126-132. doi: 10.3967/bes2022.017
  Objective   Traditional epidemiological studies have shown that C-reactive protein (CRP) is associated with the risk of cardiovascular diseases (CVDs). However, whether this association is causal remains unclear. Therefore, Mendelian randomization (MR) was used to explore the causal relationship of CRP with cardiovascular outcomes including ischemic stroke, atrial fibrillation, arrhythmia and congestive heart failure.   Methods   We performed two-sample MR by using summary-level data obtained from Japanese Encyclopedia of Genetic association by Riken (JENGER), and we selected four single-nucleotide polymorphisms associated with CRP level as instrumental variables. MR estimates were calculated with the inverse-variance weighted (IVW), penalized weighted median and weighted median. MR-Egger regression was used to explore pleiotropy.   Results   No significant causal association of genetically determined CRP level with ischemic stroke, atrial fibrillation or arrhythmia was found with all four MR methods (all Ps > 0.05). The IVW method indicated suggestive evidence of a causal association between CRP and congestive heart failure (OR: 1.337, 95% CI: 1.005–1.780, P = 0.046), whereas the other three methods did not. No clear pleiotropy or heterogeneity were observed.   Conclusions   Suggestive evidence was found only in analysis of congestive heart failure; therefore, further studies are necessary. Furthermore, no causal association was found between CRP and the other three cardiovascular outcomes.
Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus
LI Jiang Shuai, HAO Yan Zhe, HOU Mei Ling, ZHANG Xuan, ZHANG Xiao Guang, CAO Yu Xi, LI Jin Ming, MA Jing, ZHOU Zhi Xiang
2022, 35(2): 133-140. doi: 10.3967/bes2022.018
  Objective  To establish a sensitive, simple and rapid detection method for African swine fever virus (ASFV) B646L gene.  Methods  A recombinase-aided amplification-lateral flow dipstick (RAA-LFD) assay was developed in this study. Recombinase-aided amplification (RAA) is used to amplify template DNA, and lateral flow dipstick (LFD) is used to interpret the results after the amplification is completed. The lower limits of detection and specificity of the RAA assay were verified using recombinant plasmid and pathogenic nucleic acid. In addition, 30 clinical samples were tested to evaluate the performance of the RAA assay.  Results  The RAA-LFD assay was completed within 15 min at 37 °C, including 10 min for nucleic acid amplification and 5 minutes for LFD reading results. The detection limit of this assay was found to be 200 copies per reaction. And there was no cross-reactivity with other swine viruses.  Conclusion  A highly sensitive, specific, and simple RAA-LFD method was developed for the rapid detection of the ASFV.
Letter to the Editor
Estimating the Effects of the COVID-19 Outbreak on the Decreasing Number of Acquired Immune Deficiency Syndrome Cases and Epidemiological Trends in China
LI Yan Yan, DING Wen Hao, BAI Yi Chun, WANG Lei, WANG Yong Bin
2022, 35(2): 141-145. doi: 10.3967/bes2022.019
Increasing Trends and Species Diversity of Nontuberculous Mycobacteria in A Coastal Migrant City−Shenzhen, China
JI Le Cai, CHEN Shuai, PIAO Wei, HONG Chuang Yue, LI Jin Li, JIANG Qi
2022, 35(2): 146-150. doi: 10.3967/bes2022.020
Case Report of Acute Peritonitis with Blood Infection of Neisseria meningitidis
XIA Xian, LYU Jing, CAI Xuan, HU Yuan Liang, HE Fei, LI Guo Ming, YANG Hong Mei
2022, 35(2): 151-154. doi: 10.3967/bes2022.021
What Should We Focus on in Sepsis Fluid Resuscitation? — A Research Based on Scientometrics and Visual Analysis
HE Xiao Jun, ZHU Xiang, MA Yue Feng, LIANG Jun
2022, 35(2): 155-160. doi: 10.3967/bes2022.022
Pollen Extract of Populus deltoides Stimulates Lung Inflammation in a Mouse Model
GUO Wei, JIANG Yu Xin, ZHAN Xiao Dong, JIANG Feng, XI Yi Long
2022, 35(2): 161-164. doi: 10.3967/bes2022.023
Association between Organophosphate Exposure and Mental Health among Rural Residents from Two Towns in China
ZHU Jian, CUI Jia Jing, GU Yu Lu, LI Jian Hui, BESELER Cheryl L., STALLONES Lorann, KAN Mu Jie, REN Shu Ping
2022, 35(2): 165-169. doi: 10.3967/bes2022.024
Caffeine Exposure Causes Immune Dysfunction and Intrauterine Growth Restriction Retardation in Rats
ZHANG Wen Zhong, SUN Na Na, HU Yang, CAO Yu, Amber Sheeks
2022, 35(2): 170-173. doi: 10.3967/bes2022.025
Maternal Perfluorinated Compound Exposure and Risk of Early Pregnancy Loss: A Nested Case-control Study
MI Xin, LIN Shi Qi, ZHANG Xiao Fen, LI Jia Jia, PEI Li Jun, JIN Feng, LIAO Qi, XIE Li Min, WEI Li Cong, HAO Chan Juan, ZHANG Ya Wei, LI Wei
2022, 35(2): 174-179. doi: 10.3967/bes2022.026