2022 Vol. 35, No. 3

2022, 35(3)
2022, 35(3): 1-2.
Original Article
Weather and Birth Weight: Different Roles of Maternal and Neonatal GPR61 Promoter Methylation
LI Zhi Yuan, GONG Yong Xiang, YANG Meng, CHAI Jian, SUN Ren Jie, LI Qin Yang, HE Ya Nan, HUANG Hui, ZHANG Ya Wei, BA Yue, ZHOU Guo Yu
2022, 35(3): 181-193. doi: 10.3967/bes2022.027
  Objectives  It is unclear whether G protein-coupled receptor 61 (GPR61) affecting body weight, plays a role in the association between birth weight and weather. This study aimed to assess the effects of prenatal weather and GPR61 on birth weight.   Methods   A total of 567 mother-newborn pairs were recruited in Houzhai Center Hospital during 2011–2012. We detected the maternal and neonatal GPR61 promoter methylation levels, and obtained meteorological and air pollution data.   Results   A positive association was observed between maternal and neonatal GPR61 methylation levels, and both of them were affected by precipitation, relative humidity (RH) and daily temperature range (DTR). Birth weight was associated negatively with RH and positively with DTR (P < 0.05). A significant association was observed between birth weight and neonatal GPR61 methylation. We observed that maternal GPR61 methylation seemed to modify associations between weather and birth weight (Pinteraction < 0.10), while neonatal GPR61 methylation mediated the effects of RH and DTR on birth weight (P < 0.05).   Conclusions   Our findings revealed the significant associations among prenatal weather, GPR61 methylation and birth weight. Maternal GPR61 methylation may modify the susceptibility of birth weight to prenatal weather conditions, while neonatal GPR61 methylation may be a bridge of the effects of prenatal RH and DTR on birth weight.
SET8 Inhibition Potentiates Radiotherapy by Suppressing DNA Damage Repair in Carcinomas
PAN Dong, DU Ya Rong, LI Rong, SHEN Ai Hua, LIU Xiao Dong, LI Chuan Yuan, HU Bu Rong
2022, 35(3): 194-205. doi: 10.3967/bes2022.028
  Objective  SET8 is a member of the SET domain-containing family and the only known lysine methyltransferase (KMT) that monomethylates lysine 20 of histone H4 (H4K20me1). SET8 has been implicated in many essential cellular processes, including cell cycle regulation, DNA replication, DNA damage response, and carcinogenesis. There is no conclusive evidence, however, regarding the effect of SET8 on radiotherapy. In the current study we determined the efficacy of SET8 inhibition on radiotherapy of tumors and the underlying mechanism.   Methods  First, we explored the radiotherapy benefit of the SET8 expression signature by analyzing clinical data. Then, we measured a series of biological endpoints, including the xenograft tumor growth in mice and apoptosis, frequency of micronuclei, and foci of 53BP1 and γ-H2AX in cells to detect the SET8 effects on radiosensitivity. RNA sequencing and subsequent experiments were exploited to verify the mechanism underlying the SET8 effects on radiotherapy.   Results  Low expression of SET8 predicted a better benefit to radiotherapy in lung adenocarcinoma (LUAD) and invasive breast carcinoma (BRCA) patients. Furthermore, genetic deletion of SET8 significantly enhanced radiation treatment efficacy in a murine tumor model, and A549 and MCF7 cells; SET8 overexpression decreased the radiosensitivity. SET8 inhibition induced more apoptosis, the frequency of micronuclei, and blocked the kinetics process of DNA damage repair as 53BP1 and γ-H2AX foci remained in cells. Moreover, RNF8 was positively correlated with the SET8 impact on DNA damage repair.   Conclusion  Our results demonstrated that SET8 inhibition enhanced radiosensitivity by suppressing DNA damage repair, thus suggesting that SET8 potentiated radiotherapy of carcinomas. As new inhibitors of SET8 are synthesized and tested in preclinical and clinical settings, combining SET8 inhibitors with radiation warrants consideration for precise radiotherapy.
The Use of Lipoprotein-Associated Phospholipase A2 in a Chinese Population to Predict Cardiovascular Events
XI Hui, CHENG Guan Liang, HU Fei Fei, LI Song Nan, DENG Xuan, ZHOU Yong
2022, 35(3): 206-214. doi: 10.3967/bes2022.029
  Objective  To explore associations between lipoprotein-associated phospholipase A2 (Lp-PLA2) and the risk of cardiovascular events in a Chinese population, with a long-term follow-up.   Methods  A random sample of 2,031 participants (73.6% males, mean age = 60.4 years) was derived from the Asymptomatic Polyvascular Abnormalities Community study (APAC) from 2010 to 2011. Serum Lp-PLA2 levels were determined by enzyme-linked immunosorbent assay (ELISA). The composite endpoint was a combination of first-ever stroke, myocardial infarction (MI) or all-cause death. Lp-PLA2 associations with outcomes were assessed using Cox models.   Results  The median Lp-PLA2 level was 141.0 ng/mL. Over a median follow-up of 9.1 years, we identified 389 events (19.2%), including 137 stroke incidents, 43 MIs, and 244 all-cause deaths. Using multivariate Cox regression, when compared with the lowest Lp-PLA2 quartile, the hazard ratios with 95% confidence intervals for developing composite endpoints, stroke, major adverse cardiovascular events, and all-cause death were 1.77 (1.24–2.54), 1.92 (1.03–3.60), 1.69 (1.003–2.84), and 1.94 (1.18–3.18) in the highest quartile, respectively. Composite endpoints in 145 (28.6%) patients occurred in the highest quartile where Lp-PLA2 (159.0 ng/mL) was much lower than the American Association of Clinical Endocrinologists recommended cut-off point, 200 ng/mL.   Conclusion  Higher Lp-PLA2 levels were associated with an increased risk of cardiovascular event/death in a middle-aged Chinese population. The Lp-PLA2 cut-off point may be lower in the Chinese population when predicting cardiovascular events.
Direct Synthesis of Bienzyme-like Carbide-derived Carbons via Mild Electrochemical Oxidation of Ti3AlC2 MAX
FANG Yan Feng, DING Xiao Teng, XU Geng Fang, GONG Shi Da, NIU Yu Sheng, YAO Zi Yu, JIN Zhao Yong, WANG Yao, XU Yuan Hong
2022, 35(3): 215-224. doi: 10.3967/bes2022.030
  Objective   To develop effective alternatives to natural enzymes, it is crucial to develop nanozymes that are economical, resource efficient, and environmentally conscious. Carbon nanomaterials that have enzyme-like activities have been extensively developed as substitutes for traditional enzymes.   Methods   Carbide-derived carbons (CDCs) were directly synthesized via a one-step electrochemical method from a MAX precursor using an ammonium bifluoride electrolyte at ambient conditions. The CDCs were characterized by systematic techniques.   Results   CDCs showed bienzyme-like activities similar to that of peroxidase and superoxide dismutase. We systematically studied the dependence of CDC enzyme-like activity on different electrolytes and electrolysis times to confirm activity dependence on CDC content. Additionally, the synthesis mechanism and CDC applicability were elaborated and demonstrated, respectively.   Conclusion   The demonstrated synthesis strategy eliminates tedious intercalation and delamination centrifugation steps and avoids using high concentrations of HF, high temperatures, and halogen gases. This study paves the way for designing two-dimensional material-based nanocatalysts for nanoenzyme and other applications.
Understanding the Choice of Sleep Arrangements and Soothing Methods and Their Associations with Sleep Problems among Children Under 3 Years Old: A Chinese Population-Based Study
FENG Wei Wei, ZHANG Yue, WANG Hui Shan, PAN Xiao Ping, JIN Xi, XU Tao, ZHANG Tong
2022, 35(3): 225-233. doi: 10.3967/bes2022.031
  Objective  This study aimed to examine the sleep arrangements and soothing methods and to assess their associations with sleep problems among children aged < 3 years in China.   Methods  A cross-sectional survey was conducted in 2019 from six provinces in China. A total of 1,195 caregivers of children aged 0–35 months were included in the study. Data on sleep arrangements, soothing methods, and sleep problems (i.e., frequent night awakenings and difficulty falling asleep) were assessed using the Brief Infant Sleep Questionnaire. The reasons for bed-sharing in sleep arrangements were recorded using a self-designed questionnaire.   Results  The bed-sharing practice was very prevalent at any age, which ranged from 69.9% to 78.3%. Most infants fell asleep while feeding or being rocked/held before age 12 months. By age 35 months, 62.4% of the children fell asleep in bed near parents. The most common reasons for bed-sharing were breastfeeding/feeding and convenience. Parental involvement when falling asleep was significantly related with frequent night awakenings and difficulty falling asleep. No association was found between bed-sharing and sleep.   Conclusion  Bed-sharing and parental involvement were very common among Chinese children aged < 3 years. Children who fall asleep with parental involvement were more likely to have sleep problems.
Letter to the Editor
Transcriptional Regulation of YP3680 by RovA and RcsAB in Yersinia pestis biovar Microtus
LIU Lei, FANG Nan, LIU Wan Bing, HE Ying Yu, LIU Yan, ZHANG Yi Quan
2022, 35(3): 234-238. doi: 10.3967/bes2022.032
An imported Genotype D Coxsackievirus B4 Strain Identified in Tianjin, China
TAN Zhao Lin, LYU Li Kun, XIE Tong, LI Li, LI Xiao Yan
2022, 35(3): 239-247. doi: 10.3967/bes2022.033
Complete Genome Analysis of A New Strain of Coxsackievirus A1 Associated with Severe HFMD in Yunnan, China
FENG Chang Zeng, ZHANG Ming, XU Dan Han, Guo Wei, SUN Hao, YANG Zhao Qing, MA Shao Hui
2022, 35(3): 248-253. doi: 10.3967/bes2022.034
CD4+ T Cell Recovery among Patients with HIV/AIDS who Received Highly Active Antiretroviral Therapy Regularly in Chongqing, China: An Ambispective Cohort Study
ZHANG Peng, YAN Xiang Yu, LI Yong Jie, ZHANG Bo, WANG Jing
2022, 35(3): 254-258. doi: 10.3967/bes2022.035
Stopping Transmission of COVID-19 in Public Facilities and Workplaces: Experience from China
WANG Jiao, YANG Wen Jing, TANG Song, PAN Li Jun, SHEN Jin, John S. Ji, WANG Xian Liang, LI Li, YING Bo, ZHAO Kang Feng, ZHANG Liu Bo, WANG Lin, SHI Xiao Ming
2022, 35(3): 259-262. doi: 10.3967/bes2022.036
Injury Morbidity between 2013 and 2018 in Hunan Province, China
WANG Wan Hui, GAO De Yue, HUA Jun Jie, NING Pei Shan, SCHWEBEL David C, HU Guo Qing
2022, 35(3): 263-269. doi: 10.3967/bes2022.037
Gene-Environment Interactions between Environmental Noise and ApoE4 Causes AD-Like Neuropathology in the Hippocampus in Male Rats
LI Wen Long, LI Yuan Yuan, LI Yu Xin, FU Yu, HE Xian Zhi, TAO Fei Yan, YOU Ruo Lan, ZHANG Ruo Yu, ZHONG Ming Qing, CHI Hui Min, ZHAI Qing Feng
2022, 35(3): 270-275. doi: 10.3967/bes2022.038
Mono-2-ethylhexyl Phthalate Promotes Migration and Invasion by Regulating the Epithelial-Mesenchymal Transition in SH-SY5Y Cells
XU Qi, ZHANG Bo, LI Xu, YE Jia Ming, HUO Chuan Yi, YIN Jian Li, ZHANG Ru Xuan, ZHAO Wei Sen, QI Wen, YE Lin
2022, 35(3): 276-281. doi: 10.3967/bes2022.039