XIONG Lu; SUN Cheng Feng; ZHANG Jing; GAO Ya Bing; WANG Li Feng; ZUO Hong Yan; WANG Shui Ming; ZHOU Hong Mei; XU Xin Ping; DONG Ji; YAO Bin Wei; ZHAO Li; PENG Rui Yun

doi:10.3967/bes2015.002

Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway

XIONG Lu, SUN Cheng Feng, ZHANG Jing, GAO Ya Bing, WANG Li Feng, ZUO Hong Yan, WANG Shui Ming, ZHOU Hong Mei, XU Xin Ping, DONG Ji, YAO Bin Wei, ZHAO Li, PENG Rui Yun

文章导航 > Biomedical and Environmental Sciences > 2015 > 28(1): 13-24

XIONG Lu, SUN Cheng Feng, ZHANG Jing, GAO Ya Bing, WANG Li Feng, ZUO Hong Yan, WANG Shui Ming, ZHOU Hong Mei, XU Xin Ping, DONG Ji, YAO Bin Wei, ZHAO Li, PENG Rui Yun. Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway[J]. Biomedical and Environmental Sciences, 2015, 28(1): 13-24. doi: 10.3967/bes2015.002

Citation:

XIONG Lu, SUN Cheng Feng, ZHANG Jing, GAO Ya Bing, WANG Li Feng, ZUO Hong Yan, WANG Shui Ming, ZHOU Hong Mei, XU Xin Ping, DONG Ji, YAO Bin Wei, ZHAO Li, PENG Rui Yun. Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway[J]. Biomedical and Environmental Sciences, 2015, 28(1): 13-24. doi: 10.3967/bes2015.002

doi: 10.3967/bes2015.002

基金项目: ?This research was supported by the National Natural Science Foundation of China (81172620)

Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway

Beijing Institute of Radiation Medicine, Beijing 100850, China

Funds: ?This research was supported by the National Natural Science Foundation of China (81172620)

摘要: Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor (NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment.
Methods 48 male Wistar rats were exposed to 30 mW/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 mW/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated.
Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits (NR1, NR2A, and NR2B) and related signaling molecules (Ca2+/calmodulin-dependent kinase II gamma and phosphorylated cAMP-response element binding protein) were examined.
Conclusion 30 mW/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity.
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Abstract: Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor (NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment.
Methods 48 male Wistar rats were exposed to 30 mW/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 mW/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated.
Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits (NR1, NR2A, and NR2B) and related signaling molecules (Ca2+/calmodulin-dependent kinase II gamma and phosphorylated cAMP-response element binding protein) were examined.
Conclusion 30 mW/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity.
- Microwave /
- Rat hippocampus /
- PC12 /
- Synaptic plasticity /
- NMDA receptor signaling pathway Biomed Environ Sci,2015 /
- 28(1):13-24

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Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway

doi: 10.3967/bes2015.002

基金项目: ?This research was supported by the National Natural Science Foundation of China (81172620)

刊出日期: 2015-01-20

关键词:

Microwave /

Rat hippocampus /

PC12 /

Synaptic plasticity /

NMDA receptor signaling pathway Biomed Environ Sci,2015 /

28(1):13-24

摘要: Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor (NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment.
Methods 48 male Wistar rats were exposed to 30 mW/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 mW/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated.
Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits (NR1, NR2A, and NR2B) and related signaling molecules (Ca2+/calmodulin-dependent kinase II gamma and phosphorylated cAMP-response element binding protein) were examined.
Conclusion 30 mW/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity.

English Abstract

Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway

Beijing Institute of Radiation Medicine, Beijing 100850, China

Funds: ?This research was supported by the National Natural Science Foundation of China (81172620)

Publish Date: 2015-01-20

Keywords:

Microwave /
Rat hippocampus /
PC12 /
Synaptic plasticity /
NMDA receptor signaling pathway Biomed Environ Sci,2015 /
28(1):13-24

Abstract: Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor (NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment.
Methods 48 male Wistar rats were exposed to 30 mW/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 mW/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated.
Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits (NR1, NR2A, and NR2B) and related signaling molecules (Ca2+/calmodulin-dependent kinase II gamma and phosphorylated cAMP-response element binding protein) were examined.
Conclusion 30 mW/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity.

Citation:

XIONG Lu, SUN Cheng Feng, ZHANG Jing, GAO Ya Bing, WANG Li Feng, ZUO Hong Yan, WANG Shui Ming, ZHOU Hong Mei, XU Xin Ping, DONG Ji, YAO Bin Wei, ZHAO Li, PENG Rui Yun. Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway[J]. Biomedical and Environmental Sciences, 2015, 28(1): 13-24. doi: 10.3967/bes2015.002