2022 Vol. 35, No. 4

2022, 35(4): 1-2.
2022, 35(4): 1-2.
Original Article
Neonatal Exposure to Propofol Interferes with the Proliferation and Differentiation of Hippocampal Neural Stem Cells and the Neurocognitive Function of Rats in Adulthood via the Akt/p27 Signaling Pathway
MIAO Hui Hui, LIU Wen Bo, JIAO Xin Hao, SHAO Ke Jie, YUAN Ying Xuan, SHA Sha, ZHANG Qi Qi, YAN Jing, SUN Yin Ying, ZHOU Cheng Hua, WU Yu Qing
2022, 35(4): 283-295. doi: 10.3967/bes2022.040
  Objective  Neonatal exposure to propofol has been reported to cause neurotoxicity and neurocognitive decline in adulthood; however, the underlying mechanism has not been established.   Methods  SD rats were exposed to propofol on postnatal day 7 (PND-7). Double-immunofluorescence staining was used to assess neurogenesis in the hippocampal dentate gyrus (DG). The expression of p-Akt and p27 were measured by western blotting. The Morris water maze, novel object recognition test, and object location test were used to evaluate neurocognitive function 2-month-old rats.   Results  Phosphorylation of Akt was inhibited, while p27 expression was enhanced after neonatal exposure to propofol. Propofol also inhibited proliferation of neural stem cells (NSCs) and decreased differentiation to neurons and astroglia. Moreover, the neurocognitive function in 2-month-old rats was weakened. Of significance, intra-hippocampal injection of the Akt activator, SC79, attenuated the inhibition of p-AKT and increase of p27 expression. SC79 also rescued the propofol-induced inhibition of NSC proliferation and differentiation. The propofol-induced neurocognition deficit was also partially reversed by SC79.   Conclusion  Taken together, these results suggest that neurogenesis is hindered by neonatal propofol exposure. Specifically, neonatal propofol exposure was shown to suppress the proliferation and differentiation of NSCs by inhibiting Akt/p27 signaling pathway.
Subchronic Oral Toxicity Evaluation of Sodium Dehydroacetate: A 90-day Repeated Dose Study in Rats
FANG Jin, LIU Hai Bo, ZHI Yuan, FENG Yong Quan, WANG Hui Ling, CUI Wen Ming, ZHANG Ji Yue, WANG Hua Li, YU Zhou, JIA Xu Dong
2022, 35(4): 296-311. doi: 10.3967/bes2022.041
  Objective  The present study was undertaken to evaluate the subchronic oral toxicity of sodium dehydroacetate (DHA-Na) and to determine the point of departure (POD), which is a critical factor in the establishment of an acceptable dietary intake.   Methods  DHA-Na was administered once daily by gavage to Sprague–Dawley rats at dose levels of 0.0, 31.0, 62.0, and 124.0 mg/kg BW per day for 90 days, followed by a recovery period of 4 weeks in the control and 124.0 mg/kg BW per day groups. The outcome parameters were mortality, clinical observations, body weights, food consumption, hematology and clinical biochemistry, endocrine hormone levels, and ophthalmic, urinary, and histopathologic indicators. The benchmark dose (BMD) approach was applied to estimate the POD.   Results  Significant decreases were found in the 62.0 and 124.0 mg/kg BW groups in terms of the body weight and food utilization rate, whereas a significant increase was found in the thyroid stimulating hormone levels of the 124.0 mg/kg BW group. Importantly, the 95% lower confidence limit on the BMD of 51.7 mg/kg BW was modeled for a reduction in body weight.   Conclusion  The repeated-dose study indicated the slight systemic toxicity of DHA-Na at certain levels (62.0 and 124.0 mg/kg BW) after a 90-day oral exposure.
Cytokine Profiles and Virological Marker Monitoring during 48 Weeks Peginterferon Alfa Treatment for HBeAg-Positive Chronic Hepatitis B
LI Ming Hui, SUN Fang Fang, CHEN Feng Xin, ZENG Zhan, LIN Yan Jie, BI Xiao Yue, YANG Liu, DENG Wen, JIANG Ting Ting, HUANG Rong Hai, YI Wei, XIE Yao
2022, 35(4): 312-321. doi: 10.3967/bes2022.042
  Objective   This study aimed to investigate whether cytokine profiles and virological markers might add value in monitoring the effects of peginterferon (PEG-IFN) therapy for hepatitis B e-antigen (HBeAg) positive chronic hepatitis B (CHB).   Methods   HBeAg positive patients with CHB were treated with PEG-IFN for 48 weeks. Clinical biochemical, and HBV serological indexes, as well as cytokines, were detected at baseline and every 12 weeks.   Results   A total of 116 patients with CHB were enrolled in this study; 100 patients completed the 48-week treatment and follow-up, of whom 38 achieved serum HBeAg disappearance, 25 achieved HBeAg seroconversion, 37 showed HBsAg decreases ≥ 1 log10 IU/mL, 9 showed HBsAg disappearance, and 8 became HBsAb positive. The cytokine levels at baseline and during treatment were similar between the HBeAg disappearance group and non-disappearance group. The disappearance of HBeAg was independently associated with HBeAg levels at weeks 12 and 24, and with the HBeAg decline at week 24 (P < 0.05). The HBsAg response was independently associated with HBsAg, the HBsAg decline, HBeAg, the HBeAg decline at week 12, and HBsAg at week 24 (P < 0.05).   Conclusion   There was no significant correlation between the response to interferon (IFN) and cytokines during PEG-IFN treatment. The changes in virological markers predicted the response to IFN after 48 weeks.
Exploration of IRES Elements within the ORF of the Coxsackievirus B3 Genome
SONG Qin Qin, LUO Xiao Nuan, SHI Bing Tian, LIU Mi, SONG Juan, XIA Dong, XIA Zhi Qiang, WANG Wen Jun, YAO Hai Lan, HAN Jun
2022, 35(4): 322-333. doi: 10.3967/bes2022.043
  Objective  This study aimed to identify internal ribosome entry sites (IRESs) in the open reading frame (ORF) of the Coxsackievirus B3 (CVB3) genome.   Methods  The sequences of P1, P2, or P3 of the CVB3 genome or the truncated sequences from each antithymocyte globulin (ATG) to the end of the P1, P2, or P3 gene were inserted into the pEGFP-N1 vector. After transfection, possible IRES-dependent green fluorescent protein (GFP)-fused proteins were detected by anti-GFP western blotting. The sequences of possible IRESs were inserted into specific Fluc/Rluc bicistronic vectors, in which the potential IRESs were determined according to the Fluc/Rluc activity ratio. Expression of Fluc and Rluc mRNA of the bicistronic vector was detected by RT-qPCR.   Results  After transfection of full length or truncated sequences of the P1, P2, or P3 plasmids, six GFP-fused protein bands in P1, six bands in P2 and nine bands in P3 were detected through western blotting. Two IRESs in VP2 (1461–1646 nt) and VP1 (2784–2983 nt) of P1; one IRES in 2C (4119–4564 nt) of P2; and two IRESs in 3C (5634–5834 nt) and 3D (6870–7087 nt) of P3 were identified according to Fluc/Rluc activity ratio. The cryptic promoter was also excluded by RT-qPCR.   Conclusion  Five IRESs are present in the CVB3 coding region.
Influence of Microcirculatory Dysfunction on Myocardial Injury after Cardiopulmonary Resuscitation
YANG Jun, DONG Gui Juan, WANG Hong Wei, ZHAO Xin, WANG Fu Jun, ZHANG Jian, GUO Shu Bin
2022, 35(4): 334-344. doi: 10.3967/bes2022.044
  Objective  This study aimed to examine the effects of microcirculatory dysfunction and 654-1 intervention after cardiopulmonary resuscitation on myocardial injury.   Methods  Landrace pigs were divided into a sham operation group (S group, n = 6), ventricular fibrillation control group (VF-C group, n = 8) and 654-1 intervention group (VF-I group, n = 8). Hemodynamics was recorded at baseline, at recovery of spontaneous circulation (ROSC), and 1 h, 2 h, 4 h and 6 h thereafter. Sidestream dark field (SDF) technology was used to evaluate and monitor the microcirculation flow index, total vessel density, perfusion vessel ratio, De-Backer score, and perfusion vessel density in animal viscera at various time points.   Results  After administration of 654-1 at 1.5 h post-ROSC, the hemodynamics in the VF-I group, as compared with the VF-C group, was significantly improved. The visceral microcirculation detected by SDF was also significantly improved in the VF-I group. As observed through electron microscopy, significantly less myocardial tissue injury was present in the VF-I group than the VF-C group.   Conclusion  Administration of 654-1 inhibited excessive inflammatory by improving the state of visceral microcirculation.
Letter to the Editor
Involvement of Stromal Interaction Molecule 1 and Its Downstream Proteins in SiO2 Particle-Induced Release of Inflammatory Mediators from Mouse Macrophage (RAW264.7) Cells
ZOU Wen Ying, HU Zhi Yong, XUE Chang Hong, LIU Yun Gang, YE Hua
2022, 35(4): 345-350. doi: 10.3967/bes2022.045
Dose-Dependent, Frequency-Dependent, and Cumulative Effects on Cardiomyocyte Injury and Autophagy of 2.856 GHz and 1.5 GHz Microwave in Wistar Rats
ZHANG Bo, ZHANG Jing, YAO Bin Wei, XU Xin Ping, WANG Hui, ZHAO Li, DONG Ji, WANG Hao Yu, TAN Sheng Zhi, PENG Rui Yun
2022, 35(4): 351-355. doi: 10.3967/bes2022.046
N-terminal 5-mer Peptide Analog P165 of Amyloid Precursor Protein Exert Antioxidant, Anti-Inflammatory and Anti-Apoptotic Effects on UVB-Irradiated HaCaT Cells
HE Li, LIN Xue Fei, CAO Kai Na, ZHU Wei
2022, 35(4): 356-360. doi: 10.3967/bes2022.047
Study of Migration and Safety Assessment of Manganese (Mn) from Food Contact Stainless-Steel Products in China
ZHANG Hong, XING Hang, LIU Zai Mei, LI Qian Yun, CHEN Shao Hong, ZHU Lei
2022, 35(4): 361-365. doi: 10.3967/bes2022.048
Detection and Quantification of Circulating Tumor Cells in Salvaged Blood in Surgical Osteosarcoma Patients: A Pilot Study from a Tertiary Medical Center
Ayixia NAWAN, ZHENG Shao Qiang, HE Xi Qiang, WANG Geng
2022, 35(4): 366-369. doi: 10.3967/bes2022.049
Relationship between Road Network Density and Cognitive Function: Results from a Cross-Sectional Study among Adults Aged 60 Years and Older in Liaoning, China
BAI Xue, WEI Jia Xin, LUAN De Chun, LIU Meng Ting, GONG Yong Hui, WU Wei, GAO Qian
2022, 35(4): 370-374. doi: 10.3967/bes2022.050
Nicotine Weakens the Osteogenic Differentiation and Immune Regulation Capabilities of Periodontal Ligament Stem Cells
ZHANG Lu Dan, CUI Shu Yue, CHENG Chen, DING Xiao Ling, DING Gang
2022, 35(4): 375-379. doi: 10.3967/bes2022.051
2022, 35(4): 380-380.